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不同NO_3~--N/NH_4~+-N配比对紫花苜蓿盛花期生长及结瘤固氮的影响
引用本文:范俊俊,刘晓静,张晓玲,齐鹏.不同NO_3~--N/NH_4~+-N配比对紫花苜蓿盛花期生长及结瘤固氮的影响[J].草原与草坪,2015(1):44-48.
作者姓名:范俊俊  刘晓静  张晓玲  齐鹏
作者单位:1. 甘肃农业大学 草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃 兰州,730070;2. 甘肃农业大学 资源与环境学院,甘肃 兰州,730070
基金项目:国家自然科学基金,甘肃省财政厅项目“外源氮素供应对紫花苜蓿产量和品质形成影响研究”资助
摘    要:在完全营养液条件下,采用砂培法,研究了NO3--N与NH4+-N两种氮素形态的7种配比(氮素水平210mg/L)对紫花苜蓿盛花期生长及结瘤固氮的影响。结果表明:各形态配比下紫花苜蓿的株高、生物量、根瘤数、根瘤重及固氮酶活性均随NO3--N/NH4+-N增大呈先增大后减小的趋势;且NO3--N/NH4+-N比例为5/3时,紫花苜蓿的株高、生物量、根瘤数、根瘤重及固氮酶活性均取得最大值,而地上部和地下部全氮含量则在5/5时最高。综合分析,当氮素水平在210mg/L时,5/3处理下紫花苜蓿生长较好,自身固氮能力较强。

关 键 词:紫花苜蓿  NO3--N/NH4%2B-N  生长  结瘤固氮

Effects of the ratios of NO3 --N/NH4 +-N on growth,nodulation and nitrogen-fixation of alfalfa at flowering stage
FAN Jun-jun,LIU Xiao-jing,ZHANG Xiao-ling,QI Peng.Effects of the ratios of NO3 --N/NH4 +-N on growth,nodulation and nitrogen-fixation of alfalfa at flowering stage[J].Grassland and Turf,2015(1):44-48.
Authors:FAN Jun-jun  LIU Xiao-jing  ZHANG Xiao-ling  QI Peng
Abstract:Under the condition of complete nutrient solution,the effect of 7 ratios of NO3 --N/NH4 +-N (the nitrogen level was 210 mg/L)on growth,nodulation and nitrogen-fixation of alfalfa were studied at flowering stage by sand culture method.The results showed that along with the increase of NO3 --N/NH 4 +-N ratio,plant height,biomass,nodule number,nodule weight and nitrogenase activity showed a trend of increasing first and then decreasing under different ratios of NO3 --N/NH 4 +-N.The plant height,biomass,nodule number,nodule weight and nitrogenase activity of alfalfa achieved the maximum at 5/3,while the total nitrogen content of aboveground and underground were highest at 5/5.Under the nitrogen level of 210 mg/L,alfalfa performed best under 5/3 treatment and the nitrogen-fixing ability was the strongest as well.
Keywords:alfalfa  ratios of NO3--N/NH4+-N  growth  nodulation and nitrogen-fixation
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