| 灌南县域肉羊源人兽共患隐孢子虫的流行病学调查及其类型分析 |
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| 引用本文： | 朱 刚,孙爱玲,陈飞飞,朴聪雁,薄学军,徐 云.灌南县域肉羊源人兽共患隐孢子虫的流行病学调查及其类型分析[J].畜牧兽医杂志,2024,43(2):76-79. |
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| 作者姓名： | 朱 刚 孙爱玲 陈飞飞 朴聪雁 薄学军 徐 云 |
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| 作者单位： | （1.灌南县李集动物防疫检疫所，江苏 灌南 222500；2. 灌南县动物检疫所；3.灌南县畜牧兽医站;4.灌南县田楼动物防疫检疫所 ） |
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| 摘 要： | 以隐孢子虫(Cryptosporidium spp.)18S rRNA为靶基因，通过巢氏PCR检测发现在采集的101份新鲜粪便样品中18份样本为阳性。不同地区羊场的隐孢子虫感染率分别为：李集镇36%(18/50),新安镇0%(0/30)、堆沟港镇0%(0/21)未检出隐孢子虫。但通过对4羊场的分析发现，有2个羊场(50%)为隐孢子虫感染阳性，且不同的羊场感染率差异显著，因此单纯的以地区来评价隐孢子虫的感染率，是值得商榷的。山羊隐孢子虫的感染率为33.3%,湖羊隐孢子虫的感染率为2%。2～6月龄的育肥羊隐孢子虫的感染率为36%,6～10月龄的育成羊(0%)。对检测为阳性的样品进行了隐孢子虫18S rRNA基因片段序列分析，发现18个样品全部为肖氏隐孢子虫(Cryptosporidium xiaoi),不存在泛在隐孢子虫(Cryptosporidium ubiquitum)。在检测隐孢子虫感染阳性的1个山羊场和1个羊湖羊场均存在肖氏隐孢子虫感染，不存在泛在隐孢子虫，更未发现肖氏隐孢子虫和泛在隐孢子虫的混合感染。目前的数据提示肖氏隐孢子虫对2～6月龄山羊(33.3%)和湖羊(2%)具有更高的...
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| 关 键 词： | 肉羊 隐孢子虫 感染 检测 |
Epidemiological Investigation and Type Analysis of Cryptosporidium Parvum, a Zoonotic Disease that Originates from Mutton Sheep in Guannan County |
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| ZHU Gang,SUN Ailing,CHEN Feifei,PIAO Congyan,BO Xuejun,XU Yun.Epidemiological Investigation and Type Analysis of Cryptosporidium Parvum, a Zoonotic Disease that Originates from Mutton Sheep in Guannan County[J].Journal of Animal Science and Veterinary Medicine,2024,43(2):76-79. |
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| Authors: | ZHU Gang SUN Ailing CHEN Feifei PIAO Congyan BO Xuejun XU Yun |
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| Abstract: | This study used Cryptosporidium spp. 18S rRNA as the target gene, and found that 18 of the 101 fresh fecal samples collected were positive through nested PCR detection. The Cryptosporidium infection rates in sheep farms in different areas were 36% (18/50) in Liji Town, 0% (0/30) in Xin''an Town, and 0% (0/21) in Duigogang Town. No Cryptosporidium was detected. However, through the analysis of 4 sheep farms, it was found that 2 sheep farms (50%) were positive for Cryptosporidium infection, and the infection rates of different sheep farms were significantly different. Therefore, the infection rate of Cryptosporidium was simply evaluated by region, is debatable. The infection rate of Cryptosporidium parvum in goats was 33.3%, and the infection rate of Cryptosporidium parvum in Hu sheep was 2%. The infection rate of Cryptosporidium parvum in fattening sheep aged 2 to 6 months was 36%, and that of growing sheep aged 6 to 10 months (0%). The Cryptosporidium 18S rRNA gene fragment sequence analysis was performed on the samples that tested positive. We found that all 18 samples were Cryptosporidium xiaoi and there was no Cryptosporidium ubiquitum. In 1 goat farm and 1 Yanghu sheep farm that tested positive for Cryptosporidium infection, there was Cryptosporidium parvum infection, but there was no Cryptosporidium ubiquiti, and no Cryptosporidium parvum and Cryptosporidium ubiquita were found. of mixed infections. Current data suggest that Cryptosporidium shooni has a higher infection rate in goats aged 2 to 6 months (33.3%) and Hu sheep (2%). These 18 samples came from two large scale sheep farms, belonging to two sheep farms in Liji Town. |
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| Keywords: | meat sheep cryptosporidium infection detection |
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