| 高良姜提取物对PC12细胞的神经保护作用 |
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| 引用本文： | 翟红莉,;王辉,;张秀丽,;蔡彩虹,;梅文莉,;戴好富.高良姜提取物对PC12细胞的神经保护作用[J].热带生物学报,2014(1):78-83. |
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| 作者姓名： | 翟红莉 ;王辉 ;张秀丽 ;蔡彩虹 ;梅文莉 ;戴好富 |
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| 作者单位： | [1]海南大学园林园艺学院,海南海口570228; [2]中国热带农业科学院热带生物技术研究所/农业部热带作物生物学与遗传资源利用重点实验室,海南海口571101; [3]滨州医学院药学院,山东滨州264003 |
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| 基金项目： | 国家科技支撑计划课题（2013BAI11B04）;2011年海南省中药现代化专项资金（2011ZY002）;2012年海南省中药现代化专项（2012ZY008）;海南省星火产业带专项（ZDXM20120093） |
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| 摘 要： | 通过建立H2O2介导的PC12细胞损伤模型;采用MTT法检测细胞存活率,利用荧光显微镜观察细胞形态;检测细胞中乳酸脱氢酶漏出率,丙二醛(MDA)含量,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GGSH-Px)活性,评价高良姜提取物对H2O2介导的PC12细胞损伤的保护作用。结果表明,高良姜提取物能明显降低细胞内乳酸脱氢酶漏出率,降低细胞内MDA含量,提高SOD和GGSH-Px的活性,且具有浓度依赖性,由此推断,高良姜提取物对H2O2介导的PC12细胞损伤有明显保护作用。
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| 关 键 词： | 高良姜 神经保护作用 PC细胞 |
Neuroprotective Effect of Extracts from Alpinia Officinarum Hance on PC12 Cells |
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| Institution: | ZHAI Hongli , WANG Hui, ZHANG Xiuli, CAI Caihong, MEI Wenli, DAI Haofu ( 1. College of Horticulture and Landscape Architecture, Hainan University, Haikou 570228, China; 2. Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Tropical Crops/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China; 3. School of Pharmaceutical Science, Medical College of Binzhou, Shandong 264003, China) |
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| Abstract: | Model of cell injury induced by H2O2 was set up to evaluate the protective effect of the extracts derived from Alpinia officinarum Hance on PC12 cells injured by H2O2.Cell survival rate was detected by the MTTmethod,and the cell morphology was observed under fluorescence microscope.The leakage rate of lactate dehydrogenase （LDH）,malonaldehyde （MDA） content,activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） in PC12 cells were analyzed when PC12 cells were treated with the extracts from A.officinarum.The extracts obviously decreased the LDH leakage rate and the MDA content,but increased activities of SOD and GSH-Px in PC12 cells in a dose-dependent manner.It is thus inferred that the extracts of A.officinarum have an obviously protective effect on PC12 cells injured by H2O2.A neuroprotective medicine might be derived from these extracts which need further study. |
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| Keywords: | Alpinia offcinarum neuroprotective effect PC12 cell |
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