| 细菌纤维素产生菌双向凝胶电泳分析技术 |
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| 引用本文： | 杨暄,;李俊,;李从发,;郝俊冉,;许文涛,;刘四新.细菌纤维素产生菌双向凝胶电泳分析技术[J].热带生物学报,2014(1):73-77. |
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| 作者姓名： | 杨暄 ;李俊 ;李从发 ;郝俊冉 ;许文涛 ;刘四新 |
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| 作者单位： | [1]海南大学食品学院,海南海口570228; [2]中国农业大学食品科学与营养工程学院,北京100083 |
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| 基金项目： | 公益性行业（农业）科研专项（200903026-6） |
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| 摘 要： | 对细菌纤维素产生菌株———葡糖酸醋杆菌菌体蛋白的提取过程、等电点的分布及纯化方法等方面进行了探索和优化。结果表明:葡糖酸醋杆菌蛋白主要分布在pI 4~7的范围内;采用超滤浓缩后进行2D Clean-up试剂盒纯化,可以获得更优的蛋白分离效果;在考马斯亮蓝G250染色条件下,有效分离出(1037±65)个蛋白点;建立了高质量葡糖酸醋杆菌蛋白的双向凝胶电泳分析技术。
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| 关 键 词： | 细菌纤维素 欧罗巴葡糖酸醋杆菌 双向电泳 蛋白质组学 |
Two-dimensional Polyacrylamide Gel Electrophoresis Techniques for Proteomic Research of Gluconacetobacter |
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| Institution: | YANG Xuan, LI Jun, LI Congfa, HAO Junran, XU Wentao, LIU Sixin ( 1. College of Food Sciences, Hainan University, Haikou 570228, China; 2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China) |
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| Abstract: | The protein extraction,isoelectric point of distribution and protein purification of the proteins of Gluconacetobacter,a stain produced bacterial cellulose,were tried and optimized to establish a set of two-dimensional polyacrylamide gel electrophoresis （2DE） techniques for proteomic analysis of Gluconacetobacter.The results showed that Gluconacetobacter proteins were mainly distributed in the range of pI 4-7.The 2D Clean-up kit purification after ultrafiltration concentration gave better protein separation effect.Under Coomassie brilliant blue G250 condition,（1037 ±65） protein points were isolated. |
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| Keywords: | Gluconacetobacter two-dimensional polyacrylamide gel electrophoresis proteomics |
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