| 进境玉米种子携带玉米褪绿斑驳病毒的检测与鉴定 |
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| 引用本文： | 龚海燕,张永江,张治宇,陈洪俊,高必达,朱水芳.进境玉米种子携带玉米褪绿斑驳病毒的检测与鉴定[J].植物病理学报,2010,40(4):426-429. |
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| 作者姓名： | 龚海燕 张永江 张治宇 陈洪俊 高必达 朱水芳 |
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| 作者单位： | 1 中国检验检疫科学研究院, 北京 100029;2 湖南农业大学, 长沙 410128;3 青岛出入境检验检疫局, 青岛 266001 |
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| 基金项目： | "十一五"国家科技支撑计划项目，国家质检总局有害生物检测鉴定和监测业务专项 |
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| 摘 要： | Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed. The ELISA results showed that the two seedlings reacted positively with antibody against Maize chlorotic mottle virus (MCMV). The positive samples were further identified by RT-PCR method and the 711 bp size target bands were amplified specifically. The similarity range of nucleotide sequence of both RT-PCR product and six MCMV coat protein genes was 97%-99%. The amino acid sequence homology was 97.88%-99.89%. Based on the above results, the virus was identified as MCMV. It was the first time that MCMV was intercepted from imported maize seeds.
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Detection and identification of Maize chlorotic mottle virus from imported maize seeds |
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| GONG Hai-yan,ZHANG Yong-jiang,ZHANG Zhi-yu,CHEN Hong-jun,GAO Bi-da,ZHU Shui-fang.Detection and identification of Maize chlorotic mottle virus from imported maize seeds[J].Acta Phytopathologica Sinica,2010,40(4):426-429. |
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| Authors: | GONG Hai-yan ZHANG Yong-jiang ZHANG Zhi-yu CHEN Hong-jun GAO Bi-da ZHU Shui-fang |
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| Institution: | 1 Chinese Academy of Inspection and Quarantine, Beijing 100029, China;2 Hunan Agricultural University, Changsha 410128, China;3 Qingdao Entry-exit Inspection and Quarantine Bureau, Qingdao 266001, China |
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| Abstract: | Imported maize seeds were planted in a quarantine greenhouse and two seedlings with chlorotic mottle symptoms were observed. The ELISA results showed that the two seedlings reacted positively with antibody against Maize chlorotic mottle virus (MCMV). The positive samples were further identified by RT-PCR method and the 711 bp size target bands were amplified specifically. The similarity range of nucleotide sequence of both RT-PCR product and six MCMV coat protein genes was 97%-99%. The amino acid sequence homology was 97.88%-99.89%. Based on the above results, the virus was identified as MCMV. It was the first time that MCMV was intercepted from imported maize seeds. |
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| Keywords: | Maize chlorotic mottle virus coat protein gene sequence analysis |
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