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| 禽流感病毒A/Duck/Shnghai/02/99(H9)HA基因的克隆及序列分析 | |
| 引用本文： | 徐晔,王恒安,姚火春,邱列群,陈家华.禽流感病毒A/Duck/Shnghai/02/99(H9)HA基因的克隆及序列分析[J].上海交通大学学报(农业科学版),2006,24(5):456-459. |
| 作者姓名： | 徐晔 王恒安 姚火春 邱列群 陈家华 |
| 作者单位： | 1. 南京农业大学,农业部,动物疫病诊断及免疫重点实验室,南京,210095 2. 上海交通大学,农业与生物学院,上海,201101 3. 上海出入境检验检疫局,上海,200135 |
| 基金项目： | 上海市科技兴农重点攻项目 |
| 摘 要： | 根据GenBank已公布的禽流感病毒H9亚型血凝素蛋白编码基因,设计了一对引物(H1和H2),RT-PCR扩增禽流感病毒A/Duck/Shanghai/02/99(H9)的HA基因,扩增片段与载体pUCm-T的连接产物转化大肠杆菌感受态细胞,重组质粒pUCm-T-HA经限制性内切酶酶切和PCR鉴定后进行测序。BlastN分析结果显示该分离株血凝素编码基因与已发表的鸭源禽流感病毒A/Duck/Shantou/1605/01(H9N2)和A/Duck/Hong Kong/Y280/97(H9N2)血凝素编码基因的核甘酸序列同源性为98%,从而从分子水平确定了该分离株为H9亚型禽流感病毒。 |
| 关 键 词： | 禽流感病毒 HA基因 RT-PCR |
| 文章编号： | 1671-9964（2006）05-0456-04 |
| 收稿时间： | 2006-04-31 |
| 修稿时间： | 2006年4月30日 |
Cloning and Sequence Analysis of HA Gene from Avian Influenza Virus A/Duck/Shanghai/02/99(H9) |
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| XU Ye,WANG Heng-an,YAO Huo-chun,QIU Lie-qun,CHEN Jia-hua.Cloning and Sequence Analysis of HA Gene from Avian Influenza Virus A/Duck/Shanghai/02/99(H9)[J].Journal of Shanghai Jiaotong University (Agricultural Science),2006,24(5):456-459. | |
| Authors: | XU Ye WANG Heng-an YAO Huo-chun QIU Lie-qun CHEN Jia-hua |
| Institution: | 1. Key Lab of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjlng Agricultural University, Nanjing 210095; 2. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101; 3. Shanghai Entry-exit Inspection and Quarantine Bureau, Shanghai 200135,China |
| Abstract: | According to the nucleotide sequences of HA gene of avian influenza virus(AIV) H9 subtype,a pair of primer(H1 and H2) was synthesized and used to amplify the HA gene of A/Duck/Shanghai/02/99(H9) by RT-PCR using genome RNA as template.The ligation product of the amplified fragment with the linear vector pUCm-T was transformed into E.coli competent cells.After identified by restrictive endonucleonase digestion and PCR amplification,the recombinant plasmid pUCm-T-HA was sequenced.The BlastN result showed that the homology of HA gene of A/Duck/Shanghai/02/99(H9) was 98% to that of A/Duck /Shantou/1605/01(H9N2) and A/Duck/Hong Kong/Y280/97(H9N2).The haemagglutinin cleavage site of this isolate is WSYIVERPSAVN,which indicates low pathogenicity of the viral strain. |
| Keywords: | AIV HA gene RT-PCR |
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