| 几种乳杆菌协同作用对产生大果沙棘籽油共轭亚油酸的影响 |
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| 引用本文： | 王振宇,孙琪.几种乳杆菌协同作用对产生大果沙棘籽油共轭亚油酸的影响[J].东北林业大学学报,2009,37(4). |
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| 作者姓名： | 王振宇 孙琪 |
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| 作者单位： | 东北林业大学,哈尔滨,150040 |
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| 基金项目： | 黑龙江省重大科技攻关项目 |
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| 摘 要： | 以乳化的沙棘(Hippophae rhamnoides L.)籽油作为底物加入到试验所制备的复合乳杆菌中,利用乳杆菌所产生的亚油酸异构酶使亚油酸(LA)转化为共轭亚油酸(CLA).通过几种轧杆菌不同配比组合的协同试验,发现不同种乳杆菌的协同转化共轭亚油酸的能力要高于单一茵种.结果表明,复合乳杆菌中植物乳杆菌:德式乳杆菌保加利亚亚种为7:3转化效果最好,其亚油酸异构酶酶活为21.36 U/mL,共轭转化率为33.38%,共轭亚油酸质量浓度为28.83g/L.
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| 关 键 词： | 复合乳杆菌 协同作用 大果沙棘籽油 亚油酸异构酶 共轭亚油酸转化率 酶活测定 |
Effect of Synergistic Actions of Several Kinds of Lactobacilli on Producing Conjugated Linoleic Acid from Sea-Buckthorn Seed Oil of Large Berry Cultivars |
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| Wang Zhenyu,Sun Qi.Effect of Synergistic Actions of Several Kinds of Lactobacilli on Producing Conjugated Linoleic Acid from Sea-Buckthorn Seed Oil of Large Berry Cultivars[J].Journal of Northeast Forestry University,2009,37(4). |
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| Authors: | Wang Zhenyu Sun Qi |
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| Institution: | Wang Zhenyu,Sun Qi(School of Forestry,Northeast Forestry University,Harbin 150040,P.R.China) |
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| Abstract: | Emulsified seed oil from large-berry cultivars of sea-buckthorn(Hippophae rhamnoides L.) was added into the compound of Lactobacillis as substrate,and linoleate isomerase produced by Bacterium lacticum was used to convert linoleic acid(LA) into conjugated linoleic acid(CLA).Under the conditions of different ratios and combinations,it was found that the compound Lactobacillis had much stronger conversion ability than that of single one.The best conversion effect was obtained under the condition that the rati... |
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| Keywords: | Composite Lactobacilli Synergistic actions Seed oil from large-berry cultivars of sea-buckthorn Linoleate isomerase Conjugated conversion ratios Enzyme activity assay |
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