| 分离和冷冻处理对牛性控精液线粒体膜电位的影响 |
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| 引用本文： | 吴治晴,秦应和,李喜和.分离和冷冻处理对牛性控精液线粒体膜电位的影响[J].中国畜牧杂志,2010,46(1). |
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| 作者姓名： | 吴治晴 秦应和 李喜和 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100193
2. 内蒙古蒙牛繁育生物技术有限公司,内蒙古呼和浩特,011517 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 采用JC-1染色结合流式细胞仪和激光共聚焦显微镜,分别对4组牛精液样品(鲜精、分离精液、普通冻精和分离冻精)的线粒体膜电位进行检测,研究分离和冷冻处理对牛精子线粒体膜电位的影响。结果表明:分离处理对A和B种公牛的精子线粒体膜电位影响不显著(P0.05),但对C种公牛影响显著(P0.05);而冷冻处理后,A、B和C种公牛的线粒体膜电位均显著变化(P0.05);且鲜精时线粒体膜电位无显著差异的A和B种公牛,分离后线粒体膜电位出现显著差异(P0.05)。当精液从采集到处理的间隔时间在24h以内时,分离处理后精子线粒体膜电位无显著性变化(P0.05),但同样时间间隔内冷冻处理后,精子线粒体膜电位显著变化(P0.05)。实验表明和分离处理相比,冷冻处理对精子线粒体膜电位的影响更为显著,且不同种公牛对分离和冷冻处理的耐受性不同。
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| 关 键 词： | 分离 冷冻 线粒体膜电位 牛 |
Influences of Sex-sorting and Cryopreservation on Mitochondrial Transmembrance Potential of Bull Sperm |
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| WU Zhi-qing,QIN Ying-he,LI Xi-he.Influences of Sex-sorting and Cryopreservation on Mitochondrial Transmembrance Potential of Bull Sperm[J].Chinese Journal of Animal Science,2010,46(1). |
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| Authors: | WU Zhi-qing QIN Ying-he LI Xi-he |
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| Institution: | WU Zhi-qing1,QIN Ying-he1,LI Xi-he2 (1.College of Animal Science , Technology,China Agricultural University,Beijing 100193 China,2.Inner Mongolia Mengniu Reproductive Biotechnology Co.,LTD,Inner Mongolia Hohot 011517,China) |
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| Abstract: | The influences of sex-sorting and cryopreservation on the mitochondrial transmembrance potential of the bull sperm were studied. Four semen samples (fresh semen, Sex-sorted semen, unsorted frozen semen and sex-sorted frozen semen) were stained by JC -1, and detected by FCM and Confocal. The results showed that there was no significant difference in sperm mitochondrial transmembrance potential between the unsorted and sex-sorted semen in bull A and B (P > 0.05), but difference in C bull (P < 0.05). The treat... |
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| Keywords: | sex-sorting cryopreservation mitochondrial transmembrance potential bull |
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