| 菲律宾蛤仔提取物提取条件优化及其体外清除自由基的作用 |
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| 引用本文： | 梁冠豪,范秀萍,江敏婷.菲律宾蛤仔提取物提取条件优化及其体外清除自由基的作用[J].安徽农业科学,2011(10):5937-5939,5965. |
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| 作者姓名： | 梁冠豪 范秀萍 江敏婷 |
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| 作者单位： | 广东海洋大学食品科技学院 |
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| 摘 要： | 目的]研究菲律宾蛤仔全脏器提取物体外对自由基的清除作用,为其综合利用提供基础。方法]分别采用邻二氮菲-Fe2＋氧化法、DPPH反应法和邻苯三酚自氧化法,检测菲律宾蛤仔提取物体外对羟自由基（HO.）、DPPH.和超氧阴离子自由基（O2-.）的清除作用。结果]以0.1 mol/L NaCl溶液为浸提液,料液比为1∶8,75℃水浴浸提100 min,提取液经透析浓缩,再以70%乙醇终浓度沉淀,提取物（RPS-0）的得率达到3.30%,总糖与可溶性蛋白含量分别为42.40%和14.40%。RPS-0对HO.和DPPH.清除作用的IC50分别为3.100、3.500 mg/ml,对O 2-.的清除率可达到39.4%。结论]菲律宾蛤仔盐提取物具有一定的体外清除自由基作用。
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| 关 键 词： | 菲律宾蛤仔 盐提取物 自由基清除率 |
Research on the Role of the Extract of the Ruditapes philippinarum in the Free Radical-scavenging |
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| Institution: | LIANG Guan-hao et al(Institute of Food Science and Technology,Guangdong Ocean University,Zhanjiang,Guangdong 524006) |
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| Abstract: | Objective]The technical basis of comprehensive utilization of the extracts from the all organs Ruditapes philippinarum for the free radical-scavenging in vitro was provided.Method]The effect of the extracts from the Ruditapes philippinarum on the scavenging of on hydroxyl the radical(HO·),the DPPH· radical and superoxide anion free radicals(O2-·) was measured with the method of phenanthroline respectively-Fe2+ oxidation,DPPH reaction and pyrogallol autoxidation.Result]The rate of the extract(RPS-0) was 3.30%,the content of total sugar and soluble protein was 42.40% and 14.40% while the NaCl of 0.1 mol/L was as extraction solution,the ratio solid to liquid was 1∶8,75 ℃ and the time of water-extracting was 100 minutes and then the extract solution was concentrated by dialysis concentrated and precipitated with the ethanol with the concentration of 70%.The IC50(the scavenging efficiency of RPS-0 on HO· and DPPH· was 3.100 and 3.500 mg/ml and the removal rate to O2-· reached 39.4%.Conclusion]The salt extract from Manila clam had some role in free radical-scavenging. |
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| Keywords: | Ruditapes philippinarum Salt extract Free radical-scavenging |
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