| 木瓜中熊果酸提取工艺的优化及含量测定 |
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| 引用本文： | 冯华,令狐昌敏,王祥培,聂明华,罗秀琼,邹孔强,王小东.木瓜中熊果酸提取工艺的优化及含量测定[J].安徽农业科学,2011(10):5763-5764,5766. |
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| 作者姓名： | 冯华 令狐昌敏 王祥培 聂明华 罗秀琼 邹孔强 王小东 |
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| 作者单位： | 贵州省遵义市药品检验所;贵阳中医学院 |
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| 基金项目： | 贵州省卫生厅科学基金项目(gzwkj20101065) |
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| 摘 要： | 目的]优化黔北产木瓜（FRUCTUS CHAENOMELIS）熊果酸的提取工艺,并测定熊果酸含量,为木瓜的开发利用及质量控制提供科学依据。方法]采用正交试验优化木瓜熊果酸的提取工艺,用HPLC法测定熊果酸含量。结果]木瓜熊果酸的最佳提取工艺为：提取时间30 min,无水乙醇用量为25.0 ml,乙醇浓度为100%,在最佳条件下提取的熊果酸含量为0.324%。熊果酸在0.650～3.250μg/ml范围内,与峰面积呈良好的线性关系（r=0.999 1）,熊果酸平均回收率为99.20%,RSD值为0.30%。结论]优选的提取工艺稳定可行,HPLC法简便、灵敏、准确,该研究为木瓜的开发利用及质量控制提供了科学依据。
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| 关 键 词： | 黔北木瓜 熊果酸 提取工艺 正交试验 质量控制 HPLC |
Optimization of Extraction Process of Ursolic Acid from FRUCTUS CHAENOMELIS and Determination of Its Content |
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| Institution: | FENG Hua et al(Zunyi Institute for Drug Control,Zunyi,Guizhou 563002) |
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| Abstract: | Objective] To optimize the extracting conditions of ursolic acid from FRUCTUS CHAENOMELIS and determine its content,so as to provide scientific evidence for the development,application and quality control of ursolic acid.Method] The extraction conditions of ursolic acid were optimized by orthogonal test,and content of ursolic acid was determined by HPLC.Result] The optimal extraction conditions were determined to be: 30 min of extraction duration,25.0 ml of ethanol amount and extraction solvent of absolute ethanol.Under the optimum process,content of ursolic acid was 0.324%.When the concentration of ursolic acid ranged from 0.650 to 3.250 μg/ml,the relationship between ursolic acid concentration and peak area was good(r=0.999 1).Mean recovery rate of ursolic acid was 99.20% with RSD value of 0.30%.Conclusion] The optimal extraction conditions were feasible,and the established HPLC method was simple,sensitive and accurate.This study provided scientific evidence for the development,application and quality control of ursolic acid. |
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| Keywords: | FRUCTUS CHAENOMELIS Ursolic acid Extraction process Orthogonal test Quality control HPLC |
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