| HPLC法鉴别真伪野菊花的研究 |
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| 引用本文： | 查付琼,胡林峰,吴金伟,何宇新.HPLC法鉴别真伪野菊花的研究[J].安徽农业科学,2011(10):5765-5766. |
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| 作者姓名： | 查付琼 胡林峰 吴金伟 何宇新 |
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| 作者单位： | 重庆太极集团;西华大学生物工程学院 |
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| 基金项目： | 西华大学天然药物研究与工程重点实验室项目(XZD0821-09-1) |
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| 摘 要： | 目的]建立HPLC法鉴别真伪野菊花（FLOS CHRYSANTHEMI INDICI）的方法,为规范中药材市场奠定基础。方法]通过测定野菊花中蒙花苷和绿原酸含量鉴别其真伪,HPLC条件：流动相为甲醇-水-冰醋酸（V∶V∶V=26∶23∶1）;检测波长为334 nm;流速为1ml/min;柱温为35℃;进样量为10μl。结果]野菊花真品中蒙花苷和绿原酸的含量分别为8.365和0.228 mg/g,野菊花伪品中这2种物质均未检出。结论]该方法方便、可行,可用来鉴别真伪野菊花药材。
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| 关 键 词： | 野菊花 真伪 鉴别 蒙花苷 绿原酸 HPLC |
Study on Identification of Genuine and Sham FLOS CHRYSANTHEMI INDICI by HPLC |
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| Institution: | ZHA Fu-qiong et al(Taiji Group of Chongqing,Chongqing 400008) |
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| Abstract: | Objective] To identify the genuine and sham FLOS CHRYSANTHEMI INDICI by HPLC,so as to provide basis for the standardization of traditional Chinese medicine market.Method] By determining the content of buddleoside and chlorogenic acid,the genuine and sham FLOS CHRYSANTHEMI INDICI were identified.HPLC conditions were: mobile phase of methanol-water-glacial acetic acid(V∶ V∶V=26∶31∶1);detection wavelength of 334 nm;flow rate of 1 ml/min,column temperature of 35 ℃ and sampling amount of 10 μl.Result] Buddleoside and chlorogenic acid were detected in genuine FLOS CHRYSANTHEMI INDICI with content of 8.365 and 0.228 mg/g,respectively,and they were not determined in sham FLOS CHRYSANTHEMI INDICI.Conclusion] The method established in this study was convenient and feasible,which could be employed to identify FLOS CHRYSANTHEMI INDICI. |
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| Keywords: | FLOS CHRYSANTHEMI INDICI Genuine and sham Identification Buddleoside Chlorogenic acid HPLC |
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