Bacillus thuringiensis cry1Ab gene confers resistance to potato againstHelicoverpa armigera (Hubner) |
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| Authors: | S K Chakrabarti A D Mandaokar A Shukla D Pattanayak P S Naik R P Sharma P A Kumar |
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| Institution: | (1) Central Potato Research Institute, 171001 Shimla, India;(2) National Research Center for Plant Biotechnology, Indian Agricultural Research Institute, 1100012 New Delhi, India |
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| Abstract: | Summary
Helicoverpa armigera is one of the important insect pests adversely affecting the yield of potatoes in India. A synthetic gene encoding the insecticidal
crystal protein (Cry1Ab) ofBacillus thuringiensis (Bt) has been introduced into five genotypes of potato usingAgrobacterium tumefaciens. Southern analysis of DNA from transgenic plants confirmed the integration and copy number of the transgene. Double-antibody
quantitative sandwich ELISA analysis demonstrated high levels of Cry1Ab protein expression in transgenic plants. Insect bioassays
on the leaves of transgenic plants showed considerable protection against the larvae ofH. armigera in terms of leaf area consumed and larval weight reduction. |
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| Keywords: | transgenic plants Solanum tuberosum L |
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