| 芸薹种UGMS标记及抗根肿病基因连锁标记的定位 |
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| 引用本文： | 敬静,陈静静,张椿雨,朴钟云.芸薹种UGMS标记及抗根肿病基因连锁标记的定位[J].中国油料作物学报,2012,34(3):233-238. |
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| 作者姓名： | 敬静 陈静静 张椿雨 朴钟云 |
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| 作者单位： | 1. 沈阳农业大学园艺学院，辽宁 沈阳， 110161；
2.华中农业大学作物遗传改良国家重点实验室，植物科学与技术学院，湖北 武汉，430070
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| 基金项目： | 国家自然科学基金，辽宁省自然科学基金，教育部高等学校博士学科点专项科研基金 |
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| 摘 要： | 为定位芸薹种抗根肿病基因的连锁标记,利用芸薹种单一基因微卫星(UGMS)标记及基因组SSR和抗根肿病(clubroot resistance,CR)基因的紧密连锁分子标记,构建了结球白菜59-1×芜菁(CR)WJ04遗传连锁图谱,对芜菁WJ04和结球白菜59-1进行根肿病抗性鉴定和连锁位点分析。结果表明:抗根肿病芜菁自交系WJ04对来自我国根肿病主要疫区的4个根肿菌生理小种2、4、7和10均具有显性抗性,而59-1则均表现为感病。UGMS标记在大白菜和芜菁亚种间的多态性比率为30.1%,低于基因组SSR的50.8%;图谱覆盖长度为1 116.2cM,包含分布在10条连锁群的59个UGMS、72个基因组SSR和4个分别与CR基因Crr1、Crr2、Crr3和CRb连锁的标记。
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| 关 键 词： | 芸薹种 单一基因微卫星标记 根肿病 遗传连锁图谱 遗传距离 |
Mapping of unigene - derived microsatellite and markers linked to clubroot resistance loci in Brassica rapa |
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| JING Jing
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CHEN Jing-jing
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ZHANG Chun-yu
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PIAO Zhong-yun.Mapping of unigene - derived microsatellite and markers linked to clubroot resistance loci in Brassica rapa[J].Chinese Journal of Oil Crop Sciences,2012,34(3):233-238. |
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| Authors: | JING Jing CHEN Jing-jing ZHANG Chun-yu PIAO Zhong-yun |
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| Institution: | 1(1.College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China; 2.National Key Lab of Crop Genetic Improvement and College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | To localize the previously developed unigene-derived microsatellite(UGMS) markers,genomic SSR markers and markers linked to the clubroot resistance(CR) loci in Brassica rapa,a genetic linkage map was constructed using a backcross population of Chinese cabbage 59-1 and CR turnip WJ04.Clubroot resistance test indicated that WJ04 had dominant resistance to four races of No.2,4,7 and 10 of Plasmordiam brassicase,while 59-1 was susceptible to all of the races.Localization results showed that the map contained 59 UGMS,72 genomic SSR and 4 markers linked to 4 different CR loci,covering a genetic distance of 1 116cM of B.rapa genome.Four markers were linked to Crr1,Crr2,Crr3 and CRb genes respectively.Polymorphism frequency of UGMS markers was 30.1% between the two subspecies of Chinese cabbage and turnip,which was lower than that of the genomic SSR(50.8%). |
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| Keywords: | Brassica rapa Unigene-derived microsatellite marker Clubroot disease Genetic linkage map Genetic distance |
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