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Determination of water-soluble and total extractable polyphenolics in biomass, necromass and decomposing plant material using near-infrared reflectance spectroscopy (NIRS) |
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| Authors: | Marie-Madeleine Coûteaux Lina Sarmiento Dimas Acevedo |
| Institution: | a Centre National de la Recherche Scientifique, Centre d'Ecologie Fonctionnelle et Evolutive, 1919 Route de Mende, F-34293 Montpellier Cedex 5, France b Instituto de Ciencias Ambientales y Ecológicas, Facultad de Ciencias, Universidad de los Andes, Mérida 5101, Venezuela c Institut de Recherche pour le Développement, IRD, LER, BP 64501, F34394 Montpellier Cedex 5, France |
| Abstract: | Near infrared reflectance spectroscopy (NIRS) was used to predict the water-soluble and total extractable polyphenolics of plant material. Different life forms (forbs, grasses, shrubs, giant rosettes), organs (leaves, stems, roots) and decomposition stages (biomass, necromass and decomposing plant material) were studied. Prediction was good, with a R2 in validation ranging from 0.91 to 0.93 and in prediction from 0.88 to 0.94. Various standard error ratios were used to assess the quality of the models, which are generally very good, being the model for predicting the water-soluble polyphenolics in the decomposing plant material the slightly less good. Because it is a cheap and rapid method, it would allow to perform a large screening for studies concerning (i) polyphenolics control on decomposition process and (ii) phenolics implication in herbivory. |
| Keywords: | Near infrared reflectance spectroscopy Polyplenols Plant material quality Decomposition |
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