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密蒙花总黄酮清除自由基活性研究
引用本文:罗堾子,孔永强,张弘,郑华,张加研,郭元亨.密蒙花总黄酮清除自由基活性研究[J].林产化学与工业,2012,32(3):97-101.
作者姓名:罗堾子  孔永强  张弘  郑华  张加研  郭元亨
作者单位:1. 中国林业科学研究院 资源昆虫研究所 云南 昆明 650224;;2. 西南林业大学 木质科学与装饰工程学院 云南 昆明 650224;;3. 景谷林化有限公司 云南 景谷 666400
基金项目:国家林业局林业公益性行业科研专项(201004028)
摘    要:为了解密蒙花总黄酮清除自由基的能力,利用紫外-可见分光光度法对比研究了密蒙花总黄酮对1,1-二苯基-2-苦基肼自由基(DPPH.)、羟基自由基(.OH)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基(ABTS+.)和超氧阴离子自由基(O2-.)的清除作用。结果表明,密蒙花总黄酮能有效清除DPPH.、.OH、ABTS+.和O2-.。当密蒙花总黄酮质量浓度达到4.8 mg/L时,对DPPH.、.OH、ABTS+.和O2-.清除率分别可达77.88%、35.42%、85.08%和56.91%。

关 键 词:密蒙花  总黄酮  自由基  清除活性

The Radical Scavenging Activity of Flavonoids from Buddleja officinalis Maxim
LUO Chun-zi,KONG Yong-qiang,ZHANG Hong,ZHENG Hua,ZHANG Jia-yan,GUO Yuan-heng.The Radical Scavenging Activity of Flavonoids from Buddleja officinalis Maxim[J].Chemistry & Industry of Forest Products,2012,32(3):97-101.
Authors:LUO Chun-zi  KONG Yong-qiang  ZHANG Hong  ZHENG Hua  ZHANG Jia-yan  GUO Yuan-heng
Institution:1(1.Research Institue of Resources Insects,Chinese Academy of Forestry,Kunming 650224,China; 2.College of Material Engineering,Southwest Forestry University,Kunming 650224,China; 3.Jinggu Chemical Engineering of Forest Products Co.Ltd.,Jinggu 666400,China)
Abstract:In order to know the free radical scavenging effects of flavonoids from Buddleja officinalis Maxim,UV-Vis spectrum was used to study the scavenging effects of flavonoids from B.officinalis Maxim on 1,1-diphenyl-2-picrylhydrazyl(DPPH ·),hydroxyl(· OH),ABTS + ·,and superoxide(O-2 ·) free radicals.The results showed that flavonoids of B.officinalis Maxim could be well used in scavenging DPPH ·,· OH,ABTS + · and O-2 ·.When the concentration of the flavonoids concentration of B.officinalis Maxim reached 4.8 mg/L,the scavenging rates could reach 77.88%,35.42%,85.08% and 56.91%,respectively.
Keywords:Buddleja officinalis Maxim  flavonoids  free radical  scavenging activity
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