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Improvement of larval rearing technique for mass seed production of snow crab <Emphasis Type="Italic">Chionoecetes opilio</Emphasis>
Authors:Takayuki Kogane  Shigeki Dan  Katsuyuki Hamasaki
Institution:(1) Obama Station, National Center for Stock Enhancement (NCSE), Fisheries Research Agency (FRA), 917-0117 Obama, Fukui, Japan;(2) Yaeyama Station, Stock Enhancement Technology Section, Ishigaki Tropical Station, Seikai National Fisheries Research Institute, FRA, 907-0451 Ishigaki, Okinawa, Japan;(3) Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Minato, 108-8477 Tokyo, Japan;(4) Present address: Yashima Station, NCSE, FRA, 761-0111 Takamatsu, Japan
Abstract:Larval rearing experiments were conducted to examine the potential for mass seed production of the snow crab Chionoecetes opilio using a total of eight 20-kL tanks. Tanks were equipped with agitators, which move the water using a rectangular blade to prevent the zoeas sinking to the bottom of the tank. Larval rearing water was treated with sodium nifurstyrenate once a week to reduce the chance of larval infection by pathogenic bacteria. Zo eas were fed with rotifers and Artemia nauplii. A total of 122 830 megalops and 16 660 first-stage crabs were produced. Thus, the potential for mass seed production of snow crab was determined. Survival rates up to the megalopal stage were high in tanks with a feeding regime that fed rotifers to larvae through an entire zoeal stage. This study also describes the fatty acid composition of snow crab larvae. It revealed that the first zoeas had a high DHA content and DHA/EPA ratio, but these values significantly decreased in the second stage zoeas and megalops. Improving the DHA content and/or DHA/EPA ratio of larvae should be important in studies on mass seed production technology of the snow crab.
Keywords:Chionoecetes opilio            DHA  EPA  feeding regime  seed production
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