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麦根腐平脐蠕孢和薏苡平脐蠕孢防治稻田稗草的生物活性和安全性
引用本文:耿锐梅,傅扬,张文明,张建萍,余柳青.麦根腐平脐蠕孢和薏苡平脐蠕孢防治稻田稗草的生物活性和安全性[J].中国水稻科学,2008,22(3):307-312.
作者姓名:耿锐梅  傅扬  张文明  张建萍  余柳青
作者单位:1.中国水稻研究所 水稻生物学国家重点实验室, 浙江 杭州 310006; 2云南省昆明市植物保护植检站, 云南 昆明 650034;3.宁夏大学 农学院, 宁夏 银川 751200
基金项目:国家高技术研究发展计划(863计划) , 浙江省自然科学基金 , 中央级公益性科研院所专项基金
摘    要:对分离自云南昆明感病稗草\Echinochloa crus galli (L.) Beauv.\]上的病原真菌麦根腐平脐蠕孢(Bipolaris sorokiniana)和薏苡平脐蠕孢(Bipolaris coicis)开展了防治稗草潜力的评价。结果显示,B.sorokiniana和B.coicis两菌株培养5 d的发酵原液对稗草根长和芽长均有很高的抑制作用,根长最高抑制率分别为99.59%和95.78%, 芽长最高抑制率分别为91.14%和82.13%。两菌株对稗草离体叶片致病性的室内试验表明,菌丝体悬浮液处理5 d后稗草叶片病情指数最高可达100%。两菌株对稗草植株防效的温室试验表明, 菌丝体悬浮液处理稗草7 d后植株下部叶片出现萎蔫症状、脱落及死亡,处理21 d后稗草最高死亡率达到82.67%和80.00%。菌株对作物的安全性试验表明,麦根腐平脐蠕孢对作物的安全性显著高于薏苡平脐蠕孢。作为生防潜力菌株,麦根腐平脐蠕孢具有更大的应用潜力。

关 键 词:麦根腐平脐蠕孢  薏苡平脐蠕孢  稗草  生物防治  
文章编号:1001-7216(2008)03-0307-06
收稿时间:1900-01-01;
修稿时间:2007年12月26

Efficacy and Safety of Bipolaris sorokiniana and Bipolaris coicis for the Control of Echinochloa crus-galli in Paddy Field
GENG Rui-mei,FU Yang,ZHANG Wen-ming,ZHANG Jian-ping,YU Liu-qing.Efficacy and Safety of Bipolaris sorokiniana and Bipolaris coicis for the Control of Echinochloa crus-galli in Paddy Field[J].Chinese Journal of Rice Science,2008,22(3):307-312.
Authors:GENG Rui-mei  FU Yang  ZHANG Wen-ming  ZHANG Jian-ping  YU Liu-qing
Abstract:Isolates of Bipolaris sorokiniana and B.coicis from diseased barnyardgrass(Echinochloa crus-galli) plants in Kunming,Yunnan Province were evaluated for their potentials against barnyardgrass as biocontrol agent.The results of bioassay showed that the 5-day culture filtrates of the two strains had high inhibitory activity to the growth of barnyardgrass,with the inhibitory rates of 99.59% and 95.78% on roots,91.14% and 82.13% on buds,respectively.And the barnyardgrass treated with mycelium suspension of the two strains showed 100% disease incidence in the laboratory experiment.In greenhouse,the barnyardgrass plants showed the defoliating of leaves,the wilting of shoots and the death of plants after 7 days treatment by the mycelium suspension from the two strains,and the mortality rate added up to 82.67% for B.sorokiniana and 80.00% for B.coicis respectively at 21 days after treatment.In addition,the crop safety test showed that B.sorokinina was safer to major crops than B.soicis and had a better application prospect as bio-agent.
Keywords:Bipolaris sorokiniana  Bipolaris coicis  Echinochloa crus-galli  rice(Oryza sativa)  biological control
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