| 对基因组原位杂交信号释译可能出现的片面性——来自一个小麦易位系(A-3)中外源遗传物质鉴定的启示 |
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| 引用本文： | 茹岩岩,张学勇,李大勇,游光霞,晏月明.对基因组原位杂交信号释译可能出现的片面性——来自一个小麦易位系(A-3)中外源遗传物质鉴定的启示[J].作物学报,2002,28(1):6-10. |
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| 作者姓名： | 茹岩岩 张学勇 李大勇 游光霞 晏月明 |
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| 作者单位： | 中国农业科学院作物品种资源研究所,农业部作物种质资源与生物技术重点实验室,北京,100081 |
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| 基金项目： | 国家自然科学基金资助项目 (3 9870 494)~~ |
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| 摘 要： | 利用基因组原位杂交(GISH)和种子醇溶蛋白电泳(A-PAGE)技术对一个可能携带有10倍体长穗偃麦草(Thinopyrum ponticum (Host) Liu & Wang)遗传物质的小麦新种质A-3进行了综合鉴定. 用普通小麦"中国春"(Triticum aesticum L. cv. Chinese Spring)基因组(ABD)DNA作探针, 拟鹅观草Pseudoroegneria stipifolia(Czern e
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| 关 键 词： | 小麦 10倍体长穗偃麦草 黑麦 罗泊逊易位 基因组原位杂交(GISH) 种子醇溶蛋白 |
| 收稿时间： | 2001-06-11 |
Risk in Explanation of GISH Signals: Enlightenment from Verification of Alien Chromatin in a Wheat Translocation Line (A-3) by GISHRU |
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| RU Yan Yan ZHANG Xue Yong , LI Da Yong YOU Guang Xia YAN Yue Ming.Risk in Explanation of GISH Signals: Enlightenment from Verification of Alien Chromatin in a Wheat Translocation Line (A-3) by GISHRU[J].Acta Agronomica Sinica,2002,28(1):6-10. |
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| Authors: | RU Yan Yan ZHANG Xue Yong LI Da Yong YOU Guang Xia YAN Yue Ming |
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| Institution: | RU Yan Yan 1 ZHANG Xue Yong 1,* LI Da Yong 1 YOU Guang Xia 1 YAN Yue Ming 2 |
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| Abstract: | Genomic in situ hybridization (GISH) and acid polyacrylamide gel electrophoresis (A PAGE) of gliadin were used to identify the alien chromatin in a novel germplasm (A 3) derived from a wheat Thinopyrum ponticum hybrid. Using genomic DNA of "Chinese Spring"( Triticum aestivum L. ABD) as probe, genomic DNA of Pseudoroegneria stipifolia (one possible genome donor of Th. ponticum, its genome is St) as block, one pair of Robertsonian translocation chromosomes were detected via GISH. An 18S 5.8S 26S rDNA locus was located on the alien arm by FISH of p Ta 71, a rDNA sequence isolated from wheat. Further analysis of GISH with genomic DNA probes of the three diploid progenitors of T. aestivum respectively indicated that the translocated chromosomes were concerning the B genome chromosomes. The result of A PAGE of gliadin showed that A 3 conveyed the 1RS specific gliadin marker Gld B3. Using rye ( Secale cereale L.R) genomic DNA as probe and common wheat genomic DNA as block, it was then proved that A 3 was a truly 1BL/1RS translocation line by GISH. The risk for making false explanation on GISH signals caused by cross hybridization between genomes was discussed. The results indicate that we must be prudent in explanation of the GISH signals and identification of alien chromosome. It is easy to draw a false conclusion if only a single technique is adopted. Multiple methods should be used to confirm the results and check with each other. |
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| Keywords: | Wheat Thinopyrum ponticum Secale cereale Robertsonian translocation GISH Gliadin |
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