Bovine herpes virus expressing envelope protein (E2) of bovine viral diarrhea virus as a vaccine candidate. |
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| Authors: | C H Kweon S W Kang E J Choi Y B Kang |
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| Institution: | National Veterinary Research and Quarantine Service, Ministry of Agriculture and Forestry, Anyang, Republic of Korea. |
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| Abstract: | The gene encoding the envelope protein (E2) of bovine viral diarrhea virus (BVDV) was expressed under the thymidine kinase (TK) promoter of Korean bovine herpesvirus 1 (BHV-1) isolate. Thymidine kinase negative (TK-) BHV-1 recombinants expressing E2 of BVDV were constructed and the expression of E2 was identified by immunofluorescence and Western blotting. Compared to wild type BHV-1, the recombinant BHV-1 had a delayed cytopathogenic effect in cells. The immunogenicity of the recombinant BHV-1 was examined in guinea pigs and cattle. Although an increase in body temperature was detected for a few days, the inoculated cattle returned to normal temperature with the development of neutralizing antibodies to BVDV. |
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