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Effect of dietary vitamin C supplementation on the oxygen consumption, ammonia-N excretion and Na/K ATPase of Macrobrachium nipponense exposed to ambient ammonia |
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| Authors: | An-Li Wang Wei-Na Wang Yue Wang Li-Xin Shang Yuan Liu Ru-Yong Sun |
| Institution: | a College of Life Science, South China Normal University, Guangzhou 510631, PR China b College of Life Science, Hebei University, Baoding 071002, PR China c Aquaculture Research Laboratory, Ocean University of Qingdao, Qingdao 266003, PR China d College of Life Science, Beijing Normal University, Beijing 100875, PR China |
| Abstract: | The 96-h LC50 of ammonia-N and the effects of dietary vitamin C on oxygen consumption, ammonia-N excretion and Na+/K+ ATPase activity of Macrobrachium nipponense exposed to ambient ammonia were investigated. The results showed that the 96-h LC50 of ammonia-N was 36.6 mg l?1 for the freshwater prawn, M. nipponense, at pH 8.0. When prawns were exposed to high ambient ammonia-N concentrations, the oxygen consumption rate increased and ammonia excretion decreased. Dietary vitamin C supplementation led to higher oxygen consumption and lower ammonia excretion. Na+/K+ ATPase activity increased with increased ambient ammonia-N exposure in the range of 0–18.3 mg l?1, and then was reduced at ambient ammonia-N 36.6 mg l?1. Na+/K+ ATPase activities of prawns fed a vitamin C-supplemented diet were significantly lower than those of prawns fed a diet which was not supplemented with vitamin C. |
| Keywords: | Macrobrachium nipponense Vitamin C Oxygen consumption Ammonia-N excretion Na+/K+ ATPase activity Ammonia-N |
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