| 一株鹅源高致病力禽流感病毒分离株血凝素基因的分析 |
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| 引用本文： | 陈化兰,于康震,步志高.一株鹅源高致病力禽流感病毒分离株血凝素基因的分析[J].中国农业科学,1999,32(2):87-92. |
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| 作者姓名： | 陈化兰 于康震 步志高 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室，哈尔滨 150001 |
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| 摘 要： | 禽流感病毒（AIV）的表面结构蛋白血凝素（HA）在决定病毒致病力、受体结合特性、宿主范围等方面起着关键作用。本研究初步鉴定为高致病力毒株的AIV鹅体分离株A/Goose/Guangdong/1/96（H5N1）的HA基因进行了克隆并测序。结果表明，这一在无胰酶条件下可在单层鸡胚成纤维细胞培养物上形成蚀斑的毒株在其HA裂解位点附近有连续6个碱性氨基酸的插入物，从而揭示了其高致病力的分子基础。进一步的序列比较发现这一毒株与1997年香港流感病毒A/Hongkong/156/97（HK/97）的HA基因核苷酸和氨基酸同源率分别高达98.0%和98.2%，且其6个糖基化位点、2个受体结合位点及裂解位点碱性氨基酸插入物的序列均完全一致，提示该毒株与HK/97具有相似的生物学特性。
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| 关 键 词： | 禽流感病毒 H5亚型 高致病力 血凝毒基因 |
| 修稿时间： | 1999-01-12 |
Molecular Analysis of Hemagglutinin Gene of a Goose Origin Highly Pathogenic Avian Influenza Virus |
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| Chen Hualan,Yu Kangzhen,Bu Zhigao.Molecular Analysis of Hemagglutinin Gene of a Goose Origin Highly Pathogenic Avian Influenza Virus[J].Scientia Agricultura Sinica,1999,32(2):87-92. |
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| Authors: | Chen Hualan Yu Kangzhen Bu Zhigao |
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| Abstract: | The
hemagglutinin (HA) of avian influenza virus (AIV) plays the key role in determining the
pathogenicity, cell receptorbinding property and host range of the virus. A goose origin AIV
A/Goose/Guangdong/1/96(H5N1) (GD/96) was confirmed as a highly pathogenic AIV (HPAIV) by
the tests of intravenous pathogenic index (IVPI) and the assay of plaque formation. The
sequence results of the HA gene cDNA revealed that there was an insertion of 6 basic amino
acids (RRRKKR) in the cleavage site between the HA1 and HA2, which was the characterization
of the H5 subtype HPAIV. When compared with the lethal A/Hongkong/156/97(H5N1) (HK/97),
there was a homology of 98.0% at the nucleotide level and 98.2% at the amino acid level.
Furthermore, no difference of nucleotide related to all the 6 potential glycosylation sites, the 2
receptorbinding sites and the basic amino acid insert within the HA existed between GD/96 and
HK/97. These results implied that the GD/96 and HK/97 had a closely related common ancestor |
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| Keywords: | Avian influenza virus H5 subtype Highly pathogenicity Hemagglutinin gene and shared the same biology properties decided by the HA |
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