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奶牛乳房炎的病原菌分离鉴定与药敏试验
引用本文:贾玉萍,万仁忠,宁召峰,粱成彪,薛莲,刘文强,赵宏坤,李庆剧,娄焕军,李玉军,陈志伟,聂和平,李长家.奶牛乳房炎的病原菌分离鉴定与药敏试验[J].畜牧与兽医,2004,36(10):2-4.
作者姓名:贾玉萍  万仁忠  宁召峰  粱成彪  薛莲  刘文强  赵宏坤  李庆剧  娄焕军  李玉军  陈志伟  聂和平  李长家
作者单位:1. 山东农业大学动物科技学院,山东,泰安,271018
2. 济南佳宝乳业公司,山东,济南,250000
基金项目:国家“乳业”重大科技研究项目 (2 0 0 2BA51 8A1 6)
摘    要:对山东济南市 5个奶牛场的 1 65份临床型和 1 4 7份隐性型乳房炎奶样进行了病原菌分离鉴定。临床型未用利福平复合新药奶样 48份 ,细菌检出率为 83 33 % ;用该药 2d内采集的奶样 1 1 7份 ,细菌检出率 60 68%。隐性乳房炎奶样细菌检出率 72 2 9%。用新研制的利福平复合新药对未用该药奶样分离菌 35株和用该药后有代表性的分离菌 40株 ,共 75株临床型分离菌进行药敏试验 ,结果 84%为高度敏感 (63/ 75) ,1 6 %为中度敏感 (1 2 / 75)。鉴定的细菌有葡萄球菌 ,链球菌 ,大肠杆菌 ,棒状杆菌 ,芽孢杆菌等。根据用药后患病奶牛的临床表现和奶样细菌分离情况 ,表明该药对奶牛乳房炎具有显著的治疗效果

关 键 词:奶牛  乳房炎  细菌分离  利福平复合新药  药敏试验
文章编号:0529-5130(2004)10-0002-03
修稿时间:2004年3月18日

Isolation,identification and medicine sensitivity test of pathogenic bacteria of dairy cow mastitis
JIA Yu-ping,WAN Ren-zhong,NING Zhao-feng,LIANG Cheng-biao,XUE Lian,LIU Wen-qian,ZHAO Hong-kun,LI Qing-ju,LOU Huan-jun,LI Yu-jun,CHEN Zhi-wei,NIE He-ping,LI Chang-jia.Isolation,identification and medicine sensitivity test of pathogenic bacteria of dairy cow mastitis[J].Animal Husbandry & Veterinary Medicine,2004,36(10):2-4.
Authors:JIA Yu-ping  WAN Ren-zhong  NING Zhao-feng  LIANG Cheng-biao  XUE Lian  LIU Wen-qian  ZHAO Hong-kun  LI Qing-ju  LOU Huan-jun  LI Yu-jun  CHEN Zhi-wei  NIE He-ping  LI Chang-jia
Institution:JIA Yu-ping~1,WAN Ren-zhong~1,NING Zhao-feng~1,LIANG Cheng-biao~1,XUE Lian~1,LIU Wen-qian~1,ZHAO Hong-kun~1,LI Qing-ju~2,LOU Huan-jun~2,LI Yu-jun~2,CHEN Zhi-wei~2,NIE He-ping~2,LI Chang-jia~2
Abstract:The Experiments for isolation and identification of pathogenic bacteria were carried out in the milk samples of 165 clinical and 147 subclinical mastitis cases in five dairy farms in Shandong Province.The clinical cases were divided into two treatments: 117 cases were treated with Lifupingfuhexinyao medicine (LFP) and 48 cases without LFP. 83.33% (40/48) cases without LFP were detected with the bacteria infection while 60.68% (71/17) cases were detected with the infection when LFP was medicined for 2 days. The bacteria were isolated from 72.29% (107/147) of subclinical mastitis cases.The medicine sensitivity tests of LFP were oprerated with 35 strains from the samples without LFP and 40 strains from the ones with LFP. The results indicated that 84% (63/75) of the strains were highly sensitive and 16% (12/75) were mildley sensitive.
Keywords:dairy cattle  mastitis  batertia  Lifupingfuhexinyao
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