| 水稻粳型亲籼系S-b座位基因型鉴定 |
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| 引用本文： | 丁效华,张泽民,曾瑞珍,李文涛,张桂权.水稻粳型亲籼系S-b座位基因型鉴定[J].中国水稻科学,2003,17(4):297-301. |
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| 作者姓名： | 丁效华 张泽民 曾瑞珍 李文涛 张桂权 |
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| 作者单位： | 华南农业大学 植物分子育种研究中心， 广东 广州 510642 |
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| 基金项目： | 国家高技术发展计划资助项目( 2 0 0 1AA2 1 1 1 81 )，广东省自然科学基金团队项目 ( 2 0 0 30 2 3) |
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| 摘 要： | S-b是控制水稻籼粳亚种间杂种不育性的基因座位之一。在该座位，台中65的基因型为Sj/Sj，广陆矮4号的基因型为Si/Si。以台中65和广陆矮4号为遗传测验种，分别与4个粳型亲籼系配组，根据紧密连锁分子标记基因型在这些组合F2群体中的偏态分离程度， 测定了这4个粳型亲籼系在F1花粉不育性S-b基因座位的基因型，结果表明，G2416-3和G2417-2-1在S-b基因座位的基因型为Si/Si，而G2605和G3004-4的基因型为Sn/Sn。比较了不同粳型亲籼系S-b基因座位等位基因遗传效应的大小，结果表明G2416-3携带的Si基因与G2417-2-1携带的Si基因之间，以及G2605携带的Sn基因与G3004 4携带的Sn基因之间没有显著差异，而Si 与Sn之间遗传效应差异极显著。
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| 关 键 词： | 水稻 粳型亲籼系 花粉不育性 复等位基因 微卫星标记 |
| 文章编号： | 1001-7216(2003)04-0297-05 |
| 收稿时间： | 1900-01-01; |
Genotypic Identification of the S-b Locus in the Indica-Compatible Japonica Lines of Rice (Oryza sativa) |
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| DING Xiao-hua,ZHANG Ze-min,ZENG Rui-zhen,LI Wen-tao,ZHANG Gui-quan.Genotypic Identification of the S-b Locus in the Indica-Compatible Japonica Lines of Rice (Oryza sativa)[J].Chinese Journal of Rice Science,2003,17(4):297-301. |
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| Authors: | DING Xiao-hua ZHANG Ze-min ZENG Rui-zhen LI Wen-tao ZHANG Gui-quan |
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| Institution: | （Plant Molecular Breeding Research Center; South China Agricul tural University; Guangzhou 510642; China） |
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| Abstract: | S-b is one of the hybrid sterility loci between indica and japonica cultivars in rice. The genotype of Taichung 65 (T65) and Guanglu’ai 4 (GLA 4) at the locus is Sj/Sj and Si/Si, respectively. Four indica-compati ble japonica lines (ICJLs), G2416-3, G2417-2-1, G2605 and G3004-4 were cros sed with testers, T65 and GLA 4, and their S-b locus genotypes were identified. The segregation of PCR-based molecular markers linked tightly with S-b locus in F2 population showed that G2416-3 and G2417-2-1 possessed Si/Si genotype, while G2605 and G3004-4 contained genotype Sn/Sn. Comparison of genetic effect among the alleles at S-b locus in four ICJLs showed no significant difference between the Si genes in G241 6-3 and G2417-2-1, and between the Sn genes in G2605 and G3004-4, but significant difference between the Si and Sn genes. |
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| Keywords: | rice indica-compatible japonica lines pollen ster ility multi-alleles microsatellite marker |
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