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| 大叶藻组织培养中外植体消毒方法的研究 | |
| 引用本文： | 刘延岭,李晓捷,崔翠菊,王娜,赛珊,李琪.大叶藻组织培养中外植体消毒方法的研究[J].农学学报,2013,3(3):54-56. |
| 作者姓名： | 刘延岭 李晓捷 崔翠菊 王娜 赛珊 李琪 |
| 作者单位： | 1. 中国海洋大学水产学院,山东青岛 266100;山东东方海洋科技股份有限公司,山东烟台 264003 2. 山东东方海洋科技股份有限公司,山东烟台,264003 3. 中国海洋大学水产学院,山东青岛,266100 |
| 基金项目： | 烟台市科技发展计划项目"人工大叶藻场构建的生物学研究" |
| 摘 要： | 为探寻大叶藻组织培养中外植体的最佳消毒方法,采用75%乙醇、1.5%碘化钾、4%次氯酸钠和10%双氧水这4种消毒剂以及不同的处理时间组合,对大叶藻种子和幼苗进行消毒试验。结果表明,最有效的外植体消毒方法为:对大叶藻幼苗,先用无菌水冲洗3～4次,再以75%乙醇处理20s,然后再用无菌水冲洗3～4次,可以达到较好的消毒效果。对大叶藻种子,先以75%乙醇处理10min,再以1.5%碘化钾处理10min,然后用无菌海水冲洗3～4次,这样消毒效果最佳,且不影响种子的发芽率。 |
| 关 键 词： | 大叶藻 组织培养 消毒方法 消毒剂 |
| 收稿时间： | 2013/1/11 0:00:00 |
| 修稿时间： | 2013/2/2 0:00:00 |
Study on the disinfection method for tissue culture of Zostera marina |
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| Liu Yanling , Li Xiaojie , Cui Cuiju , Wang Na , Sai Shan , Li Qi.Study on the disinfection method for tissue culture of Zostera marina[J].Journal of Agriculture,2013,3(3):54-56. | |
| Authors: | Liu Yanling Li Xiaojie Cui Cuiju Wang Na Sai Shan Li Qi |
| Institution: | 1 (1 College of Fisheries,Ocean University of China,Qingdao 266100,Shandong,China;2 Shandong Oriental Ocean Sci-tech Co.Ltd,Yantai 264003,Shandong,China) |
| Abstract: | In order to establish the suitable disinfection method for tissue culture of Zoster marina. 75% ethanol, 1.5% potassium iodide, 4% sodium hypochlorite and 10% hydrogen peroxide as well as different disinfection time were used for seeds and seedlings sterilization treatment. The results indicated that for seedlings, sterile sea water rinsed for 3-4 times, 75% ethanol sterilized for 20 s, then sterile sea water rinsed for 3-4 times, which achieved good sterilizing effects. The efficient disinfection method for seeds was that, 75% ethanol rinsed for 10 min, 1.5% potassium iodide immersed for 10 min and then sterile sea water rinsed for 3-4 times, which showed the optimal disinfection effects and did not affect seed germination rate. |
| Keywords: | disinfectant |
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