| 鸭新城疫病毒和鸭圆环病毒二重PCR检测方法的建立 |
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| 引用本文： | 许宗丽,谢芝勋,谢丽基,刘加波,谢志勤,邓显文,范 晴.鸭新城疫病毒和鸭圆环病毒二重PCR检测方法的建立[J].中国动物检疫,2012,29(5):34-37. |
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| 作者姓名： | 许宗丽 谢芝勋 谢丽基 刘加波 谢志勤 邓显文 范 晴 |
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| 作者单位： | 1. 广西大学,动物科学技术学院,广西南宁530004／广西壮族自治区兽医研究所广西畜禽疫苗新技术重点实验室,广西南宁530001
2. 广西壮族自治区兽医研究所广西畜禽疫苗新技术重点实验室,广西南宁,530001 |
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| 基金项目： | 广西科技项目，广西特聘专家专项经费，国家百千万人才工程人选专项资金项目，桂兽科09-2共同资助 |
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| 摘 要： | 本研究根据GenBank中鸭新城疫病毒(NDV)的F基因和鸭圆环病毒(DuCV)的V1/rep基因的保守序列,各设计一对特异性引物,并对二重PCR的扩增条件进行优化,建立了鸭NDV和DuCV的二重PCR检测方法。对混合样品进行扩增,得到2条大小为493bp(鸭NDV)和218bp(DuCV)的特异性条带,与预扩增片段相符。而对番鸭细小病毒、鸭瘟病毒、鸭肝炎病毒、鸭源小鹅瘟病毒、鸭H9亚型流感病毒、鸭疫里氏杆菌、大肠杆菌、禽多杀性巴氏杆菌等病原检测,结果为阴性。该方法的敏感性试验表明,鸭NDV的核酸最小量为40fg,DuCV为20fg。
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| 关 键 词： | 鸭新城疫病毒 鸭圆环病毒 二重PCR |
Development of a Duplex PCR Assay for Detection of Duck Newcastle Disease Virus and Duck CircovirusDuck |
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| Xu Zongli,Xie Zhixun,Xie Liji,Liu Jiabo,Xie Zhiqin,Deng Xianwen,Fan Qing.Development of a Duplex PCR Assay for Detection of Duck Newcastle Disease Virus and Duck CircovirusDuck[J].China Journal Of Animal Quarantine,2012,29(5):34-37. |
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| Authors: | Xu Zongli Xie Zhixun Xie Liji Liu Jiabo Xie Zhiqin Deng Xianwen Fan Qing |
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| Institution: | 1.College of Veterinary Medicine,Guangxi University,Nanning 530004,China;2.Guangxi Veterinary Research Institute,Guangxi Key Laboratory of Animal Vaccines and New Technology,Nanning 530001,China) |
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| Abstract: | According to the sequences of duck NDV F gene and DuCV V1/rep gene in GenBank,two pairs of specific primers were designed,and the reaction conditions were optimized,and then a duplex PCR assay was developed for detection of Newcastle disease virus and circovirus in ducks.All samples containing Newcastle disease virus and circovirus could be amplified into two specific bands,493 bp for duck Newcastle disease virus and 218 bp for duck circovirus by this duplex PCR,but no specific bands of the same sizes were amplified from other duck pathogens,such as Muscovy duck parvovirus,duck plague virus,duck hepatitis virus,gosling plague virus,duck H9 subtype avian influenza virus,Riemerella anatipestifer,E.coli,avian Pasteurella multocida.As little as 40 fg of duck NDV and 20 fg of DuCV DNA could be detected. |
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| Keywords: | Duck Newcastle disease virus Duck circovirus Duplex PCR |
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