| 八肽胆囊收缩素对骨髓间充质干细胞诱导分化后神经元样细胞生长状态的影响 |
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| 引用本文： | 王丽,何涛,于海清,李娟,段承刚.八肽胆囊收缩素对骨髓间充质干细胞诱导分化后神经元样细胞生长状态的影响[J].四川农业大学学报,2010,28(2):241-244. |
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| 作者姓名： | 王丽 何涛 于海清 李娟 段承刚 |
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| 作者单位： | 泸州医学院,医学分子生物学实验室,四川,泸州,646000 |
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| 基金项目： | 四川省科技厅重点科技项目 |
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| 摘 要： | 为探讨八肽胆囊收缩素(CCK8)对骨髓间充质干细胞(MSCs)诱导分化后神经元样细胞生长状态的影响,在β-巯基乙醇诱导MSCs分化的基础上加入CCK8,观察诱导期间细胞形态变化及分化细胞的存活时间,免疫细胞化学法鉴定神经元特异性巢蛋白(Nestin)的表达,唑盐比色实验(MTT)法检测细胞活性。结果表明,加入CCK8的部分实验组与基础诱导组相比,可延长神经元样细胞的生存时间;诱导后5 h,Nestin阳性表达率变化不明显(P>0.05),诱导后10 h,神经元样细胞的活性可明显增强(P<0.05)。该结果说明,CCK8在β-巯基乙醇体外诱导大鼠MSCs分化为神经元样细胞后的过程中,可一定程度延长细胞的存活时间,具有抗细胞凋亡作用。
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| 关 键 词： | 八肽胆囊收缩素 骨髓间充质干细胞 神经元 分化 |
Effect of Octa-cholecystokinin on the Survival of Neuron-like Cells Differentiated from Mesenchymal Stem Cells |
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| WANG Li,HE Tao,YU Hai-qing,LI Juan,DUAN Chen-gang.Effect of Octa-cholecystokinin on the Survival of Neuron-like Cells Differentiated from Mesenchymal Stem Cells[J].Journal of Sichuan Agricultural University,2010,28(2):241-244. |
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| Authors: | WANG Li HE Tao YU Hai-qing LI Juan DUAN Chen-gang |
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| Abstract: | To investigate the effect of octapeptide cholecystokinin(CCK8) on the survival of neuron like cells differentiated from mesenchymal stem cells(MSCs) in vitro,the MSCs were induced with β-mercaptoethanol(β-ME) and different concentration CCK8 since pre-induction.Morphological changes and survival time of induced MSCs were observed during cell differentiation and survival.ABC immunocytochemical detection was used for the expression of Neuron-specific marker-Nestin and MTT was used to observe the activity of neuron-like cells.As a result,CCK8 was able to extend cell survival time in some experiment groups.Though CCK8 seemed to be able to improve the activity of neuron-like cells as well as the expression of Nestin,no statistic significance was confirmed when induced 5 hours later(P>0.05).The activity of neuron-like cells was significant difference with induction group after 10 hours later(P<0.05).The results suggested that CCK8 could improve the survival of neuron-like cells differentiated from MSCs in vitro and has the anti apoptosis effect. |
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| Keywords: | octapeptide cholecystokinin mesenchyrnal stem cells neuron differentiation |
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