| 葡萄糖氧化酶产生菌发酵条件优化研究 |
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| 引用本文： | 赵勇,宋志文,孔小蓉.葡萄糖氧化酶产生菌发酵条件优化研究[J].安徽农业科学,2010(25):13582-13583,13585. |
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| 作者姓名： | 赵勇 宋志文 孔小蓉 |
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| 作者单位： | 青岛理工大学环境与市政工程学院,山东青岛,266033;青岛理工大学环境与市政工程学院,山东青岛,266033;青岛理工大学环境与市政工程学院,山东青岛,266033 |
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| 摘 要： | 目的]探讨黑曲霉发酵产葡萄糖氧化酶的最佳发酵条件。方法]研究不同培养基成分及发酵条件下黑曲霉产葡萄糖氧化酶的活性。结果]培养基最佳成分为及浓度为：葡萄糖100g/L,有机氮源为4g/L蛋白胨,无机氮源为3g/L硝酸钠;最佳发酵条件为28℃,pH值6,发酵周期72h。结论]通过优化,黑曲霉发酵产葡萄糖氧化酶的活性明显提高。
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| 关 键 词： | 葡萄糖氧化酶 发酵培养基 碳源 氮源 发酵条件 |
Studies on Fermentation Conditions Optimization of Bacteria Producing Glucose Oxidas |
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| ZHAO Yong et al.Studies on Fermentation Conditions Optimization of Bacteria Producing Glucose Oxidas[J].Journal of Anhui Agricultural Sciences,2010(25):13582-13583,13585. |
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| Authors: | ZHAO Yong |
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| Institution: | ZHAO Yong et al (Environmental , Municipal Engineering College,Qingdao Technological University,Qingdao,Sh,ong 266033) |
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| Abstract: | Objective]The aim of the study was to explore the optimum fermentation conditions of Aspergillus niger glucose oxidase.Method]Aspergillus niger glucose oxidase activity under different medium composition and fermentation conditions was investigated.Result]The medium and the concentration of the best ingredients was that glucose was 100 g/L,organic nitrogen source was peptone of 4 g/L,inorganic nitrogen source was sodium nitrate of 3 g/L;The optimum fermentation conditions were 28 ℃,pH 6,72 h fermentation period.Conclusion]Through the optimization,the Aspergillus niger glucose oxidase activity increased significantly. |
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| Keywords: | Glucose oxidase Fermentation medium Carbon source Nitrogen Fermentation conditions |
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