| 番茄愈伤组织诱导与细胞悬浮培养方法的改良 |
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| 引用本文： | 姜云斌,郭红莲,陈嘉,孙明杰.番茄愈伤组织诱导与细胞悬浮培养方法的改良[J].安徽农业科学,2008,36(8):3134-3135. |
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| 作者姓名： | 姜云斌 郭红莲 陈嘉 孙明杰 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津,300457;天津科技大学食品工程与生物技术学院,天津,300457;天津科技大学食品工程与生物技术学院,天津,300457;天津科技大学食品工程与生物技术学院,天津,300457 |
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| 基金项目： | 天津科技大学食品工程与生物技术学院资助项目. |
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| 摘 要： | 目的]探讨番茄愈伤组织及细胞悬浮培养的最佳培养条件。方法]用番茄的子叶作为外植体,研究不同浓度2,4-D与6-BA组合诱导"美国红"番茄子叶愈伤组织形成、不定芽发生的情况。方法]在MS基本培养基上附加不同水平6-BA和2,4-D的培养基中,愈伤组织形态发生的情况不同,其中激素含量起主要作用。子叶外植体以MS+2,4-D0.05mg/L+6-BA0.01mg/L为最佳再生培养基,对愈伤组织的形成和不定芽的分化都有良好的效果。不同生长调节物质对细胞的悬浮培养有一定的影响,以2,4-D0.2mg/L+NAA0.2mg/L+ZT0.05mg/L的浓度组合为最佳。结论]该研究确认了番茄愈伤组织及细胞悬浮培养的最佳培养条件,为番茄组织培养奠定了基础。
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| 关 键 词： | 愈伤组织 悬浮细胞培养 2 4-D 6-BA |
| 文章编号： | 0517-6611(2008)08-03134-02 |
| 修稿时间： | 2007年11月13 |
Improvement on Methods of Tomato Callus Induction and Cell Suspension Culture |
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| JIANG Yun-bin.Improvement on Methods of Tomato Callus Induction and Cell Suspension Culture[J].Journal of Anhui Agricultural Sciences,2008,36(8):3134-3135. |
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| Authors: | JIANG Yun-bin |
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| Abstract: | Objective] The aim was to study the optimum culture conditions of tomato callus induction and cell suspension culture. Method] Taking tomato cotyledon as explants, the effects of different concentration combinations with 2,4-D and 6-BA on callus formation and differentiation of adventitious buds were studied. Result] MS medium added to different concentration 2,4-D and 6-BA had different effects on callus formation. Thereinto, hormones played a major role. The best medium of callus induction was MS 2,4-D 0. 05 mg/ L 6-BA 0. 01 mg/ L, which had good effects on callus formation and differentiation of adventitious buds. The different hormones had some effects on cell suspension culture, and the best combination of concentration was 2,4-D 0.2 mg/ L NAA 0.2 mg/ L ZT 0.05 mg/ L. Conclusion] The study confirmed the optimum culture conditions of tomato callus induction and cell suspension culture, and provided basis for tomato tissue culture. |
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| Keywords: | Callus Cell suspension culture 2 4-D 6-BA |
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