| 白菜离体春化相关基因表达的cDNA-AFLP分析 |
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| 引用本文： | 孙保娟,曹家树,黄细松,余小林.白菜离体春化相关基因表达的cDNA-AFLP分析[J].园艺学报,2006,33(6):1341-1344. |
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| 作者姓名： | 孙保娟 曹家树 黄细松 余小林 |
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| 作者单位： | ( 浙江大学蔬菜研究所, 浙江杭州310029; 广东省农业科学院蔬菜研究所, 广东广州510640) |
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| 基金项目： | 浙江省重大科技攻关项目(2005C12019-02)，国家自然科学基金项目(30370975) |
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| 摘 要： | 借助cDNA-AFLP技术分离到15个与白菜离体春化反应相关的表达序列标签( EST) 。Blast结果表明: 低温春化处理抑制性表达的12个EST, 多与异化作用相关基因同源性较高; 春化诱导表达的3个EST, 分别与编码核糖体蛋白的亚基(或与自主开花途径相关的FAC基因连锁的因子) , ATPase的亚基,小G - 蛋白亚基的基因同源性较高。
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| 关 键 词： | 白菜 春化 离体 基因 cDNA-AFLP |
| 文章编号： | 0513-353X（2006）06-1341-04 |
| 收稿时间： | 2006-01-12 |
| 修稿时间： | 2006-01-122006-03-27 |
cDNA-AFLP Analysis on Genes Associated with Vernalization in Vitro in Brassica campestris ssp. chinensis |
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| Sun Baojuan,Cao Jiashu,Huang Xisong,Yu Xiaolin.cDNA-AFLP Analysis on Genes Associated with Vernalization in Vitro in Brassica campestris ssp. chinensis[J].Acta Horticulturae Sinica,2006,33(6):1341-1344. |
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| Authors: | Sun Baojuan Cao Jiashu Huang Xisong Yu Xiaolin |
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| Institution: | ( Institute of Vegetable Science, Zhejiang University, Hangzhou, Zhejiang 310029, China; Vegetable Research Inseitute, Guangdong Academy of Agricultural Sciences, Guangzhou, Guangdong 510640, China) |
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| Abstract: | Fifteen ESTs associated with vernalization in vitro of Chinese cabbage-pak-choi(Brassica campestris L.ssp.chinensis Makino)were isolated by cDNA-AFLP.Blast results showed that 12 ESTs downregulated by vernalization had high similarity to genes encoding enzymes involved in the disassimilation.3 ESTs upregulated had high similarity to genes with positive effect on vernalization,including ribosomal protein subunit related to the synthesis of priotein or the linkage factor of FAC involved in auto flowering pathway,the subunit of ATPase,and the subuint of small-G-protein. |
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| Keywords: | Chinese cabbage-pak-choi Brassica campestris ssp chinensis Vernalization In vitro Gene cDNA-AFLP |
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