| 吉氏巴贝斯虫cDNA探针的制备及杂交试验 |
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| 引用本文： | 陈启军,李德昌.吉氏巴贝斯虫cDNA探针的制备及杂交试验[J].中国动物检疫,1995,12(2):8-10. |
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| 作者姓名： | 陈启军 李德昌 |
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| 作者单位： | 长春解放军农牧大学动物医学系!长春，130062，长春解放军农牧大学动物医学系!长春，130062，长春解放军农牧大学动物医学系!长春，130062 |
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| 摘 要： | 本实验将－６．６ｋｂ的吉氏巴贝斯虫ｃＤＮＡ片段以光照活化法标记光敏生物素，制备成光敏生物素探针。与吉氏巴贝斯虫基因组ＤＮＡ、伊氏锥虫基因组ＤＮＡ、犬白细胞ＤＮＡ的斑点杂交试验表明，该探针可与０．００１ｎｇ以上量的吉氏巴贝斯虫ＤＮＡ杂交，而不与任何浓度的伊氏锥虫ＤＮＡ和犬ＤＮＡ杂交，具有很高的敏感性和特异性。
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| 关 键 词： | 吉氏巴贝斯虫 犬病 DNA探针 |
Preparation and hybridization Test of a Babesia gibsoni cDNA Probe |
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| Chen QiJun el al.Preparation and hybridization Test of a Babesia gibsoni cDNA Probe[J].China Journal Of Animal Quarantine,1995,12(2):8-10. |
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| Authors: | Chen QiJun el al |
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| Abstract: | A 6. 6kb cDNA insert fragment was subcloned from a cDNA library of Babesia gibsoni andlabbelled with photobiotin to prepare a biotinylated cDNA probe. Results of bybridization test of thispch with1,0. 1,0. 01,0. 001ng B.gibsoni kenomic DNA showed that it could, detect 0. 001ng B. gibsoni genomic DNAand did not hybridize with Trypanosoma evansi DNA and dog leucocyte DNA. The results indicated that thisphotobiotin-labelled B. gibsoni cDNA probe had provided the most sensitive and specific method for the diagnosis and epidemic investigation of B. gibsoni infection. |
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| Keywords: | Babesia gibsoni cDNA probe hybridization |
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