| Impacts of Environmental Factors on Degradation of CrylAb Insecticidal Protein in Leaf-Blade Powders of Transgenic Bt Rice |
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| 作者姓名： | BAI Yao-yu JIANG Ming-xing CHENG Jia-an |
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| 作者单位： | [1]Institute of Insect Sciences, Zhejiang University, Hangzhou 310029, P.R.China [2]Chongqing Key Laboratory of Entomology ＆ Insect Control Engineering~College of Plant Protection, Southwest University, Chongqing 400716, P.R.China |
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| 基金项目： | This study was financially supported by the National Natural Science Foundation of China (30070500) to Cheng Jiaan. |
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| 摘 要： | The determination of the environmental fate of Bt insecticidal protein released by Bt rice plants in paddy soils is a key issue in its ecological risk assessment. In this study, the impacts of soil water content, pH, and temperature on the degradation of CrylAb protein expressed in the leaves of Bt rice KMD2 were studied in the laboratory. Three types of paddy soils were used, i.e., blue clayey paddy soil, pale paddy soil on quaternary red soil, and marine-fluvigenic yellow loamy paddy soil. Ground powders of KMD2 leaf blades were mixed with each type of soil, and degradation dynamics of Cry lAb were measured using enzyme-linked immunosorbent assay （ELISA）. The degradation rate of CrylAb was high at the early experimental stage, but slowed down steadily at middle and later stages, which could be described by exponential equations, with the half-life period of degradation determined as 1.8-4.0 d. The soil water content, pH, and temperature could affect the degradation of CrylAb, but the effects of soil pH and temperature were relatively greater. In general, CrylAb degradations were slower under lower soil pH and temperature conditions, especially for marine-fluvigenic yellow loamy paddy soil.
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| 关 键 词： | Bt转基因稻 叶片粉 CrylAb杀虫蛋白 降解作用 环境因子 水稻土 生物安全性 |
Impacts of Environmental Factors on Degradation of Cry 1 Ab Insecticidal Protein in Leaf-Blade Powders of Transgenic Bt Rice |
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| BAI Yao-yu JIANG Ming-xing CHENG Jia-an.Impacts of Environmental Factors on Degradation of CrylAb Insecticidal Protein in Leaf-Blade Powders of Transgenic Bt Rice[J].Agricultural Sciences in China,2007,6(2):167-174. |
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| Authors: | BAI Yao-yu Ph D Associate Professor JIANG Ming-xing CHENG Jia-an Professor |
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| Institution: | aInstitute of Insect Sciences, Zhejiang University, Hangzhou 310029, P.R.China;bChongqing Key Laboratory of Entomology & Insect Control Engineering/College of Plant Protection, Southwest University, Chongqing 400716, P.R.China |
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| Abstract: | The determination of the environmental fate of Bt insecticidal protein released by Bt rice plants in paddy soils is a key issue in its ecological risk assessment. In this study, the impacts of soil water content, pH, and temperature on the degradation of Cry1Ab protein expressed in the leaves of Bt rice KMD2 were studied in the laboratory. Three types of paddy soils were used, i.e., blue clayey paddy soil, pale paddy soil on quaternary red soil, and marine-fluvigenic yellow loamy paddy soil. Ground powders of KMD2 leaf blades were mixed with each type of soil, and degradation dynamics of Cry1Ab were measured using enzyme-linked immunosorbent assay (ELISA). The degradation rate of Cry1Ab was high at the early experimental stage, but slowed down steadily at middle and later stages, which could be described by exponential equations, with the half-life period of degradation determined as 1.8-4.0 d. The soil water content, pH, and temperature could affect the degradation of Cry1Ab, but the effects of soil pH and temperature were relatively greater. In general,Cry 1 Ab degradations were slower under lower soil pH and temperature conditions, especially for marine-fluvigenic yellow loamy paddy soil. |
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| Keywords: | transgenic Bt rice CrylAb insecticidal protein paddy soil degradation environmental factors |
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