| 1株乳酸生产菌的筛选及发酵培养基的优化 |
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| 引用本文： | 崔东波,苑广志.1株乳酸生产菌的筛选及发酵培养基的优化[J].安徽农业科学,2011,39(12):6953-6955,6958. |
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| 作者姓名： | 崔东波 苑广志 |
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| 作者单位： | 辽宁农业职业技术学院,辽宁营口,115009 |
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| 摘 要： | 目的]为乳酸菌的发酵生产奠定基础。方法]从14株耐碱微生物中筛选出1株性能优良的产乳酸菌LP3-3,并通过单因素试验对该菌发酵生产乳酸的培养基进行优化。结果]产乳酸菌LP3-3经16S rRNA序列测定初步鉴定为金橙黄微小杆菌（Exiguobacteriumaurantiacum）。经优化,产乳酸菌LP3-3培养基中最佳碳源是葡萄糖,最佳氮源是酵母膏,酵母膏用量为1%,碳氮比为5。确定了其最佳发酵培养基组成：葡萄糖50.0 g/L,酵母膏10.0 g/L,蛋白胨4.0 g/L,乙酸钠（无水）2.0 g/L,柠檬酸三铵2.0 g/L,K2HPO4.H2O 2.0g/L,MgSO4.7H2O 0.2 g/L,MnCl2o4H2O 0.05 g/L,吐温80 1 ml/L,NaCl 40 g/L,初始pH为9.0。结论]通过培养基的优化提高了产乳酸菌LP3-3的活力。
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| 关 键 词： | 乳酸生产菌 筛选 发酵培养基 |
Screen of the Bacterial Strains for Lacti Acid Production and Optimization of its Fermentation Medium |
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| CUI Dong-bo et al.Screen of the Bacterial Strains for Lacti Acid Production and Optimization of its Fermentation Medium[J].Journal of Anhui Agricultural Sciences,2011,39(12):6953-6955,6958. |
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| Authors: | CUI Dong-bo |
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| Institution: | CUI Dong-bo et al (Liaoning Agriculture Vocational Technical College,Yingkou,Liaoning 115009) |
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| Abstract: | Objective] The basis of the lactic acid fermentation was laid.Method The bacterial strain LP3-3,which had a higher yield of lactic acid,was obtained from 14 alkali-resistant microorganisms.The medium for the LP3-3 fermentation was optimized with single-factor experiment.Results] The results indicated that the LP3-3 was confirmed to be Exiguobacterium aurantiacum C/N based on the preliminary identification of 16S rRNA sequencing.The optimal experiment in its fermentation medium indicated the glucose was u... |
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| Keywords: | Bacterial strain producing lacti acid Screen Fermentation medium |
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