| 原位吸附提高葡萄悬浮培养中反式白藜芦醇产量的研究 |
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| 引用本文： | 岳向国,张卫,邓麦村.原位吸附提高葡萄悬浮培养中反式白藜芦醇产量的研究[J].安徽农业科学,2011,39(6):3223-3226,3236. |
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| 作者姓名： | 岳向国 张卫 邓麦村 |
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| 作者单位： | 中国科学院大连化学物理研究所海洋生物产品工程组,辽宁大连,116023;中国科学院研究生院,北京,100039;澳大利亚弗林德斯大学医学院医学生物技术系分子加工及生物产品实验室,阿德莱德,SA,5042;中国科学院研究生院,北京,100039 |
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| 摘 要： | 目的]研究原位吸附对葡萄悬浮培养体系生长和生产的影响。方法]考察5种常用吸附剂对葡萄细胞生长及芪类和花色苷生产的影响。并对筛选出的HP2MGL和XAD-7的最佳添加浓度进行了研究。结果]在添加浓度为100 g/L的XAD-7和HP2MGL的处理组中反式白藜芦醇的产量最高,在第7天达到251.4和258.8 mg/L,将白藜芦醇苷和白藜芦醇的产量换算成总芪产量（mmol/L）,结果显示,添加XAD-7和HP2MGL后,在第7天芪类产量达到最大,分别为1.4和1.5 mmol/L,比对照处理的芪类产量高27%。在筛选出XAD-7和HP2MGL后,又考察了添加不同浓度XAD-7和HP2MGL对葡萄细胞生长、花青素和芪类生产的影响。结果表明最佳优化结果为添加200 g/L的HP2MGL,此时,体系中反式白藜芦醇在第7天的产量为568 mg/L。结论]200 g/L HP2MGL大孔树脂可以显著促进葡萄悬浮培养细胞体系中反式白藜芦醇产量。
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| 关 键 词： | 细胞悬浮培养 葡萄 反式白藜芦醇 |
Study on Enhancing Production of Trans-resveratrol in Vitis vinifera Cell Suspension Cultures by in Situ Absorption |
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| Institution: | YUE Xiang-guo et al(Marine Bioproducts Engineering Group,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian Liaoing,116023) |
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| Abstract: | Objective] The aim was to research the effect of in situ adsorption in the the Vitis vinifera suspension cell culture.Method] The effect of five kinds of resins on the anthocyanin,trans-piceid and trans-resveratrol production of Vitis vinifera cell suspension culture were investigated,and the best addition concentration of HP2MGL and XAD-7which were selected was studied.Result] The results showed that the maximum production of trans-resveratrol(251.4 and 258.8 mg/L) and the maximum production of total stilbenes(1.4 and 1.5 mmol/L) occurred on the seventh day when 100 g/L XAD-7 and HP2MGL was added respectively.Afterwards,the effect of addition of different concentration(100,200 and 300 g/L) of XAD-7 and HP2MGL were investigated,and the maximum production of trans-resveratrol(568 mg/L) was reached on the seventh day,when the 200 g/L HP2MGL was introduced.Conclusion] The production of trans-resveratrol in Vitis vinifera cell suspension culture could be enhanced highly by 200 g/L HP2MGL in situ absorption. |
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| Keywords: | Cell suspension culture Vitis vinifer Trans-resveratrol |
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