| 半滑舌鳎组织器官中一氧化氮含量和一氧化氮合酶活性的测定 |
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| 引用本文： | 张胜根,邢克智,孙敬锋,王庆奎,马倩倩.半滑舌鳎组织器官中一氧化氮含量和一氧化氮合酶活性的测定[J].安徽农业科学,2011,39(12):7295-7297. |
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| 作者姓名： | 张胜根 邢克智 孙敬锋 王庆奎 马倩倩 |
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| 作者单位： | 天津农学院水产科学系,天津市水产生态及养殖重点实验室,天津,300384 |
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| 基金项目： | 天津市应用基础及前沿技术研究计划项目，教育部重点科研项目，天津农学院青年科研基金项目 |
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| 摘 要： | 目的]研究半滑舌鳎不同组织器官中一氧化氮（NO）含量和不同类型一氧化氮合酶（NOS）活性。方法]采用Griess试剂法测定NO含量,采用化学比浊法测定NOS的活性。结果]诱导型一氧化氮合酶（iNOS）在肌肉组织中活性最高,为28.774±4.10 U/mgprot,依次为肠、脑、肝脏、头肾、鳃、中肾、脾脏、心脏。肠和脑之间以及肝脏和头肾之间iNOS的活性不存在显著性差异,而其他组织器官之间iNOS的活性都存在显著性差异。结构型一氧化氮合酶（cNOS）在肠组织中活性最高,为55.979±5.048 U/mgprot。所测组织器官中cNOS活性都存在显著性差异。NO含量在脾脏中最高,为38.540±4.535μmol/L。脾脏、头肾、肝脏之间,以及脑、心脏、中肾之间NO含量不存在显著性差异,而其他组织器官之间NO含量都存在显著性差异。结论]不同组织器官中NO含量和NOS活性不同,这可能与组织器官功能有着密切联系。
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| 关 键 词： | 半滑舌鳎 组织器官 一氧化氮 一氧化氮合酶 |
Determination of Nitric Oxide Production and Nitric Oxide Synthase Activity in Organizations of Half-smooth Tongue-sole (Cynoglossus semilaevis Gunther) |
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| ZHANG Sheng-gen et al.Determination of Nitric Oxide Production and Nitric Oxide Synthase Activity in Organizations of Half-smooth Tongue-sole (Cynoglossus semilaevis Gunther)[J].Journal of Anhui Agricultural Sciences,2011,39(12):7295-7297. |
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| Authors: | ZHANG Sheng-gen |
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| Institution: | ZHANG Sheng-gen et al(Tianjin Key Lab of Aqua-Ecology and Aquaculture,Department of Fishery Science,Tianjin Agricultural University,Tianjin 300384) |
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| Abstract: | Objective] The aim was to study nitric oxide production and nitric oxide synthesis activity in the organizations of Cynoglossus semilaevis Gunther.Method] Griess Reagent was used to determinate the nitric oxide(NO) production,and the method of chemical colorimetry was used to measure the nitric oxide synthase(NOS) activity.Result] Inducible NOS(iNOS) activity was the highest in the muscle(28.774±4.10 U/mgprot),followed by the intestine,brain,live,head-kidney,sill,kidney,spleen,and heart.Statistical analysis showed that there were no significant differences in iNOS activity between gut and brain or between liver and head kidney;however,there were significant differences in iNOS activity among other organizations.The constitutive NOS(cNOS) activity was highest in the intestine(55.979±5.048 U/mgprot).Statistical analysis showed that there were significant differences in cNOS activity among measured organizations.The spleen was found to have the highest level of NO production(38.540±4.535 μmol/L).Statistical analysis showed that there were no significant differences in NO production among spleen,head kidney and liver or among brain,heart and kidney;however,there were significant differences in NO production among other organizations.Conclusion] Different level of NO production and NOS activity existed in the different organizations of Cynoglossus semilaevis,which probably was related with the functions of the organizations. |
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| Keywords: | Cynoglossus semilaevis Gunther Organizations Nitric oxide Nitric oxide synthase |
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