| 亮甲酚蓝染色对水牛卵母细胞体外受精和孤雌激活胚胎发育的影响 |
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| 引用本文： | 黄怡,陈焕华,刘宏毅,张明,陆阳清,卢克焕.亮甲酚蓝染色对水牛卵母细胞体外受精和孤雌激活胚胎发育的影响[J].安徽农业科学,2011,39(7):4004-4006. |
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| 作者姓名： | 黄怡 陈焕华 刘宏毅 张明 陆阳清 卢克焕 |
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| 作者单位： | 1. 广西大学动物繁殖研究所,广西亚热带生物资源保护利用重点实验室,广西 南宁 530004;广西桂林市妇女儿童医院,广西 桂林 541000
2. 广西大学动物繁殖研究所,广西亚热带生物资源保护利用重点实验室,广西 南宁 530004 |
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| 基金项目： | 国家高技术研究发展计划(863计划)项目，广西科学研究与技术开发项目 |
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| 摘 要： | 目的]探讨亮甲酚蓝（BCB）染色对水牛卵母细胞体外受精及孤雌激活胚胎发育的影响。方法]卵丘卵母细胞复合体（COCs）在体外成熟培养前用浓度26μmol/L的亮甲酚蓝染色90 min,然后根据卵母细胞胞质蓝色的有无分为阳性（BCB＋,蓝色）和阴性组（BCB-,不着色）。以未经染色处理的COCs为对照组,控制对照组卵母细胞在含0.4%牛血清白蛋白（BSA）的杜氏磷酸盐缓冲液（DPBS）中孵育90 min。体外成熟培养后统计各组的成熟率、体外受精和孤雌激活后的发育率。结果]BCB＋组的成熟率（65.70%）显著高于对照组（59.86%）、控制对照组（58.42%）和BCB-组（48.86%）。在体外受精后的囊胚发育率方面,BCB＋组（27.08%）和对照组（25.49%）差异不显著,但显著高于控制对照组（20.50%）和BCB-组（8.45%）。在孤雌激活后的囊胚发育率方面,BCB-组显著低于其他各组,但BCB＋组、对照组以及控制对照组之间无显著差异。结论]亮甲酚蓝染色筛选出的阳性卵母细胞具有较好的核成熟发育潜能,但并不能显著提高卵母细胞体外受精及孤雌激活后的胚胎发育率。
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| 关 键 词： | 水牛卵母细胞 体外受精 孤雌激活 亮甲酚蓝 |
Effects of Brilliant Cresyl Blue Staining on the Efficiency of in vitro fertilizatin and Parthenogenetic Activation of Oocytes in Buffalo |
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| Institution: | HUANG Yi et al(Animal Reproduction Institute,Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization,Guangxi University,Nanning,Guangxi 530004) |
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| Abstract: | Objective] This paper aims to evaluate the effects of brilliant cresyl blue(BCB) staining on the efficiency of in vitro fertilization(IVF)and parthenogenetic activation(PA)of oocytes in buffalo.Method] Cumulus oocyte complexes(COCs) are stained with BCB(26 μmol/L) for 90 min before in vitro maturation(IVM).Subsequently,COCs are divided into BCB-group(colorless cytoplasm) and BCB+ group(colored cytoplasm) according to their ooplasm BCB coloration.The COCs without BCB staining are considered as control group,and the COCs of holding control group are incubated in DPBS supplemented with 0.4% BSA for 90 min.After IVM,the maturation rate,embryo development rate of IVF and PA in each group are evaluated.Result] The maturation rate in BCB+ group(65.70%) is significantly higher than that of control group(59.86%),holding control group(58.42%) and BCB-group(48.86%).The blastocyst rate of IVF in BCB + group(27.08%) is significantly higher than that of holding control group(20.50%) and BCB-group(8.45%),but there is no significant difference between the BCB+ group and the control group(27.08% vs 25.49%).There is no significant difference in parthenogenetic blastocyst rate among the BCB + group,control group and holding control group(19.13%),but all of the three groups are significantly higher than that of BCB-group.Conclusion] The positive buffalo COCs selected by BCB staining before IVM have superior developmental competence in maturation,but it could not significantly enhance the developmental rate of the IVF and PA embryos. |
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| Keywords: | Buffalo oocyte IVF PA Brilliant cresyl blue |
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