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| 渗透胁迫对玉米幼苗类囊体膜上结合态多胺含量的影响 | |
| 引用本文： | 刘怀攀,胡炳义,刘天学,李潮海.渗透胁迫对玉米幼苗类囊体膜上结合态多胺含量的影响[J].华北农学报,2007,22(2):86-89. |
| 作者姓名： | 刘怀攀 胡炳义 刘天学 李潮海 |
| 作者单位： | 1. 河南农业大学,农学院,作物生长与发育调控省重点实验室,河南,郑州,450002;周口师范学院,植物学重点实验室,河南,周口,466000 2. 周口师范学院,植物学重点实验室,河南,周口,466000 3. 河南农业大学,农学院,作物生长与发育调控省重点实验室,河南,郑州,450002 |
| 基金项目： | 科技部科技攻关项目 , 中国博士后科学基金 |
| 摘 要： | 研究了渗透胁迫下,玉米品种农大108(抗旱性较强)和掖单13(抗旱性较弱)幼苗类囊体膜上非共价结合态(Noncovalently conjugated,NCC)多胺(Polyamines,PAs)和高氯酸不溶性共价结合态(Perchloric acid insoluble covalently con-jugated,PISCC)多胺(PA)含量的变化。发现渗透胁迫下,抗旱性弱的掖单13的NCC-亚精胺(Spermidine,Spd)、PISCC-腐胺(Putrescine,Put)和PISCC-Spd下降幅度明显大于抗旱性强的农大108;掖单13的NCC-Put含量明显上升,NCC-精胺(Spermine,Spm)含量明显下降,而农大108的NCC-Put和NCC-Spm含量变化不明显。外源Spd处理明显抑制了渗透胁迫下掖单13类囊体膜上NCC-Spd含量的下降,也提高了掖单13玉米幼苗的抗性;Spd生物合成的专一性抑制剂——MGBG处理,则明显促进了渗透胁迫下农大108类囊体膜上NCC-Spd含量的下降,也降低了农大108玉米幼苗的抗性。PISCC-PAs生物合成的专一性抑制剂——o-phen处理,促进了渗透胁迫下玉米幼苗类囊体膜上PISCC-PAs含量的下降,而且明显降低了玉米幼苗的抗性。表明玉米幼苗类囊体膜上NCC-Spd、PISCC-Spd和PISCC-Put在胁迫条件下的稳定有利于增强玉米幼苗适应渗透胁迫的能力。 |
| 关 键 词： | 玉米 结合态多胺 渗透胁迫 类囊体膜 |
| 文章编号： | 1000-7091（2007）02-0086-04 |
| 修稿时间： | 2006-10-28 |
Effect of Osmotic Stress on the Content of Conjugated PA to the Thylakoid Membrane of Maize Seedlings |
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| LIU Huai-pan,HU Bing-yi,LIU Tian-xue,LI Chao-hai.Effect of Osmotic Stress on the Content of Conjugated PA to the Thylakoid Membrane of Maize Seedlings[J].Acta Agriculturae Boreali-Sinica,2007,22(2):86-89. | |
| Authors: | LIU Huai-pan HU Bing-yi LIU Tian-xue LI Chao-hai |
| Institution: | 1. Henan Key Laboratory for Regulating Controlling Crop Growth and Development, Henan Agricultural University, Zhengzhou 450XE,China; 2. Key Laboratory of Plant Science, Zhoukou Normal University, Zhoukou 466000,China |
| Abstract: | The changes in contents of noncovalently conjugated-polyamines(NCC-Pas) and perchloric acid insoluble covalently conjugated-polyamines(PISCC-Pas) to thylakoid membrane were investigated in seedling leaves of two maize(Zea mays L.) cultivars,Nongda 108(drought-tolerant) and Yedan 13(drought-sensitive) under osmotic stress.On the 7th day after polyethylene glycol(PEG)-6 000 treatment, the contents of NCC-spermidine(Spd),PISCC-putrescine(Put) and PISCC-Spd decreased more significantly in Yedan 13 than in Nongda 108.In Yedan 13,the contents of NCC-Put and NCC-spermine(Spm) decreased more significantly,while Nongda 108 showed unaltered levels in both of them.Under osmotic stress,exogenous Spd treatment inhibited the decrease of NCC-Spd level in Yedan 13 and enhanced its tolerance to stress.Treatment with ethylglyoxal-bis(guanylhydrazone)(MGBG),which is an inhibitor of Spd biosynthesis,enhanced the decrease of NCC-Spd level in Nongda 108 and decreased its tolerance to stress.Treatment with o-phen,an inhibitor of PISCC-PAs biosynthesis,enhanced the decrease of PISCC-PAs and decreased the tolerance of seedlings to stress.The results suggested that the stability of NCC-Spd,PISCC-Put and PISCC-Spd contents in thylakoid membrane could enhance the adaptation of maize seedlings to osmotic stress. |
| Keywords: | Maize Conjugated PA Osmotic stress Thylakoid membrane |
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