| 植物表达载体pGMAR-BADH的构建 |
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| 引用本文： | 朱永兴,许兴,陈受宜.植物表达载体pGMAR-BADH的构建[J].中国农学通报,2007,23(11):49-51. |
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| 作者姓名： | 朱永兴 许兴 陈受宜 |
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| 作者单位： | 1. 宁夏农业生物技术重点实验室,银川,750002
2. 中国科学院遗传与发育研究所,北京,100101 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 将甜菜碱醛脱氢酶基因BADH和植物表达载体pGMAR通过内切酶BamHI和KpnI双酶切,T4连接酶进行连接反应。重组质粒在大肠杆菌菌株DH5α内扩增,提取并纯化质粒。经鉴定,基因BADH已被完整、正确的插入到pGMAR载体中,并成功将BADH插入到载体pGMAR的两个MAR序列之间。
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| 关 键 词： | 甜菜碱醛脱氢酶基因 大肠杆菌 限制性核酸内切酶 电泳 |
| 修稿时间： | 2007-07-18 |
Construction of the Plant Expression Vector pGMAR- BADH |
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| Zhu Yonxing,Xu Xing,Chen Shouyi.Construction of the Plant Expression Vector pGMAR- BADH[J].Chinese Agricultural Science Bulletin,2007,23(11):49-51. |
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| Authors: | Zhu Yonxing Xu Xing Chen Shouyi |
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| Institution: | The key lab of agricultural bio-technology of Ninxia, Yinchuan 750002, 2Institute of genetics and developmental biology Chinese Academy of Science, Beijing 100101 |
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| Abstract: | Both the plant expression vector PGMAR and the Salt-tolerant Correlative Gene BADH in Atriplex were digested by BamHI and KpnI.Two digested fragments were ligated with T4DNA ligase and PGMAR-BADH plasmid was constructed.After the identification of digesting and sequencing,the reconstructive plasmid was confirmed that contained the correct and full nucleotides sequence of BADH in PGMAR. |
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| Keywords: | Betaine aldehyde dehydrogenase Escherichin coli restriction enzyme electrophoresis |
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