| 羊草幼穗离体培养诱导植株再生的研究 |
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| 引用本文： | 刘公社,汪恩华,刘杰,齐冬梅,李芳芳.羊草幼穗离体培养诱导植株再生的研究[J].草地学报,2002,10(3):198-202. |
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| 作者姓名： | 刘公社 汪恩华 刘杰 齐冬梅 李芳芳 |
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| 作者单位： | 中国科学院植物研究所, 北京, 100093 |
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| 基金项目： | Key Project of the Chinese Academy of Science ( KSCX1-0 8-0 3) and State Key Project of theMinistry of Science and Technology( 2 0 0 1BA70 7B0 2 ) |
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| 摘 要： | 采用离体培养方法首次获得羊草体细胞再生植株。其方法要点是:取羊草幼穗为外植体,经0.1%HgCl2溶液表面消毒后,接种到含2mg/L2,4-D的MS培养基上,置于恒温25℃条件下诱导愈伤组织。在加有1mg/L2,4-DMS培养基上继代2次后,转移到含1.0mg/LKT和0.5mg/LNAA的MS培养基上分化培养得到再生芽。在无激素的基本培养基上获得了生根的试管苗,试管苗移栽到温室后生长正常。研究结果还表明,尽管从羊草叶片、幼穗和成熟胚在同样培养条件下均能诱导出愈伤组织,但只有幼穗愈伤组织能够继续分化出再生植株。羊草试管苗的分化因基因型和外源激素的不同而异。
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| 关 键 词： | 羊草(Leymus chinensis) 幼穗 外殖体 再生植株 |
| 收稿时间： | 2002-01-31 |
Plant Regeneration of Leymus chinensis Via in Vitro Culture |
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| LIU Gong-she,WANG En-hua,LIU Jie ,QI Dong-mei,LI Fang-fang.Plant Regeneration of Leymus chinensis Via in Vitro Culture[J].Acta Agrestia Sinica,2002,10(3):198-202. |
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| Authors: | LIU Gong-she WANG En-hua LIU Jie QI Dong-mei LI Fang-fang |
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| Institution: | Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China |
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| Abstract: | The regenerated plants were obtained from Leymus chinensis for the first time through tissue culture.Immature-base explants were cultured on Murashige and Skoog's basal medium(MS) supplement-ed with 2 mg/L 2,4-D after surface sterilized with 0.1% HgCl2 to induce callus at 25℃ constant temperature.After moved to MS medium containing 1mg/L 2,4-D for two times,the compact calluses were selected and transferred to MS basal medium containing 1.0mg/L Kt and 0.5mg/L NAA,which differentiated into shoot-buds. The shoot-buds appeared and regenerated new roots,finally developed plantlets on 2,4-D-free basal medium. Regenerated plants growth well when transferred into greenhouse.A comparative study of different explants,in this study,revealed that only could the callus from immature stachys differentiate into regenerated plants. The differentiate potential of regenerated plants has close related to the genotype and external hormone. |
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| Keywords: | Leymus chinensis Immature stachys Explant Plant regeneration |
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