| 灭菌乳中氨基酸测定条件改进 |
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| 引用本文： | 翁霞,辛广,梁文涛.灭菌乳中氨基酸测定条件改进[J].乳业科学与技术,2012,35(2):38-40. |
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| 作者姓名： | 翁霞 辛广 梁文涛 |
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| 作者单位： | 鞍山师范学院化学与生命科学学院,辽宁鞍山,114007 |
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| 基金项目： | 鞍山师范学院院级课程建设项目 |
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| 摘 要： | 考察茚三酮比色法测定灭菌乳中氨基酸条件的主要影响因素。通过单因素及正交试验对其测定条件进行改进。结果表明：水浴时间对测定结果影响显著,要严格控制。茚三酮比色法测定灭菌乳中氨基酸的最佳条件为：水浴时间30min、水浴温度70℃、茚三酮用量1.2mL,在此条件下测得灭菌乳中氨基酸含量为1.62g/100mL。
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| 关 键 词： | 灭菌乳 氨基酸 测定条件 |
Optimization of Ninhydrin Reaction Conditions for Determination of Amino Acid Content in Sterilized Milk |
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| WENG Xia,XIN Guang,LIANG Wen-tao.Optimization of Ninhydrin Reaction Conditions for Determination of Amino Acid Content in Sterilized Milk[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2012,35(2):38-40. |
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| Authors: | WENG Xia XIN Guang LIANG Wen-tao |
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| Institution: | (College of Chemistry and Life Sciences, Anshan Normal University, Anshan 114007, China) |
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| Abstract: | One-factor-at-a-time and orthogonal array design methods were used to optimize the major reaction conditions affecting the determination of amino acid content in sterilized milk by ninhydrin colorimetric method.Water-bath heating time influenced significantly determination results so that it required strict control.The optimal determination conditions were 1.2 mL of ninhydrin solution and heating for 30 min in 70 ℃ water-bath.Under these conditions,the amino acid content in sterilized milk was determined to be 1.62 g/100 mL. |
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| Keywords: | sterilized milk amino acid determination conditions |
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