| 激光共聚焦显微镜的样品前处理技术研究 |
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| 引用本文： | 王志颖,饶玉春,刘敏.激光共聚焦显微镜的样品前处理技术研究[J].安徽农业科学,2013(26):10602-10603,10621. |
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| 作者姓名： | 王志颖 饶玉春 刘敏 |
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| 作者单位： | 浙江师范大学化学与生命科学学院,浙江金华,321004;浙江师范大学化学与生命科学学院,浙江金华,321004;浙江师范大学化学与生命科学学院,浙江金华,321004 |
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| 基金项目： | 浙江师范大学2013年度实验技术开发项目(sj201314) |
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| 摘 要： | 激光共聚焦显微镜是近代生物医学图像应用中最重要的仪器之一,在定量共聚焦图像分析、定量荧光测量、三维重组分析生物结构等方面具有重要的作用.该研究主要介绍了实验室中最常用的激光共聚焦显微镜的几种样品前处理技术,即FM4-64染色法、BFA处理、双分子荧光互补技术（BiFC）等,同时介绍这些方法的原理、使用范围以及在相关领域中的应用情况等,以期更好地将激光共聚焦显微镜技术应用于科研实践中.
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| 关 键 词： | 激光共聚焦显微镜 样品 前处理 |
Research of Test Sample Pretreatment Technology in Confocal Laser Scanning Microscopy |
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| Institution: | WANG Zhi-ying(College of Chemistry ＆ Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang 321004) |
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| Abstract: | Confocal laser scanning microscopy is one of the most important instruments in modern biomedical imaging applications.It plays an important role in quantitative confocal image analysis,quantitative fluorescence measurements,three-dimensional analysis of biological structure reorganization,and so on.The most commonly used sample preparation techniques of confocal laser scanning microscopy in laboratory were introduced：FM4-64 staining,BFA treatment,complementary bimolecular fluorescence technique （BiFC）,etc..The principle of these methods,using scope,and the application in the relevant field were introduced,so as to better use the laser confocal microscope in scientific research. |
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| Keywords: | Confocal laser scanning microscopy Sample Pretreatment |
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