| 动植物总核酸提取试验 |
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| 引用本文： | 刘文,柯辉鹏,梁红.动植物总核酸提取试验[J].仲恺农业技术学院学报,2008,21(3). |
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| 作者姓名： | 刘文 柯辉鹏 梁红 |
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| 作者单位： | 仲恺农业工程学院,生命科学学院,广东,广州,510225 |
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| 摘 要： | 依据最简单的核酸提取3步骤:细胞裂解、去除杂质、沉淀核酸的原理设计了一种普适性的动植物核酸提取方法(简称PS法),并对包括裸子植物门的银杏纲、苏铁纲、松柏纲,被子植物门的双子叶植物纲和单子叶植物纲的20种植物材料以及动物界的5纲5种材料进行了提取试验.结果表明,用PS方法提取动植物总核酸均能取得较理想的提取效果.所提取的总核酸分别用RNase和DNase进行消化,可分离纯化出高纯度的DNA和RNA.
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| 关 键 词： | 核酸提取 DNA RNA PS法 |
A broad-suitable method for total nucleic acid extraction from plants and animals |
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| LIU Wen,KE Hui-peng,LIANG Hong.A broad-suitable method for total nucleic acid extraction from plants and animals[J].Journal of Zhongkai Agrotechnical College,2008,21(3). |
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| Authors: | LIU Wen KE Hui-peng LIANG Hong |
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| Abstract: | According to simple three steps for nucleic acid extraction,in cluding cells rupturing,impurities removing and nucleic acid precipitation a new nucleic acid extraction method called PS method was worked out.Better results were obtained on the basis of extractions from 20 species of plants including Ginkgopsida,Cycadinae,Coniferae,dicots and monocotyledon of angiosperms,and 5 species of animals of 5 classes.High purity of DNA and RNA were obtained after digestion with DNase and RNase respectively.The extracted DNA and RNA were excellent for RT-PCR and SSR amplifications.The experimental results showed that the PS method was a broad-suitable method for nucleic acid extraction from plants and animals. |
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| Keywords: | nucleic acid extraction DNA RNA PS method |
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