| 柠条锦鸡儿根瘤菌及其共生固氮基因多样性 |
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| 引用本文： | 张红娟,张朝阳,强 磊.柠条锦鸡儿根瘤菌及其共生固氮基因多样性[J].西北农业学报,2014,23(10):194-199. |
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| 作者姓名： | 张红娟 张朝阳 强 磊 |
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| 作者单位： | ( 1.杨凌职业技术学院，陕西杨凌 712100；2.旱区作物逆境生物学国家重点实验室，西北农林科技大学，陕西杨凌 712100) |
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| 基金项目： | 陕西省科学技术研究发展计划项目（2011K01-20）；杨凌职业技术学院科学研究基金项目（A2012033）。 |
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| 摘 要： | 为研究西北干旱地区豆科植物柠条锦鸡儿(Caragana korshinskii)根瘤菌的系统发育地位及共生基因多样性,对从甘肃省分离得到的菌株进行16SrRNA PCR-RFLP和序列分析,构建系统发育树,确定其分类地位,并通过分析共生基因nodA和nifH,确定种群共生类型。结果表明:从柠条根瘤内分离到64株菌,共有7个PCR-RFLP 16SrRNA基因型,主要归属于Ensifer(占分离总数43.8%)、Mesorhizobium(31.2%)、Rhizobium(25%),其中,Ensifer为主要类群,约占34.4%。共生基因nodA和nifH与Ensifer和Mesorhizobium模式菌株同源性较高,与16SrRNA基因确定的根瘤菌分类地位相比,发现部分共生基因与16S rRNA基因确定的分类地位不同,揭示了共生基因横向转移现象的发生。
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| 关 键 词： | 柠条 根瘤菌 共生基因 系统发育 PCR-RFLP |
Diversity of Rhizobia and Symbiotic Genes Associated with Caragana korshinskii |
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| ZHANG Hongjuan,ZHANG Chaoyang and QIANG Lei.Diversity of Rhizobia and Symbiotic Genes Associated with Caragana korshinskii[J].Acta Agriculturae Boreali-occidentalis Sinica,2014,23(10):194-199. |
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| Authors: | ZHANG Hongjuan ZHANG Chaoyang and QIANG Lei |
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| Abstract: | To study the diversity of symbionts and phylogenetic position associated with Caragana korshinskii in arid and semi-arid region of northwester China, we performed the analysis of 16S rRNA sequences and constructed the phylogenetic tree for the isolated strains, and then determined the symbiotic type by analyzing the nodA and nifH gene sequences. 64 strains were isolated from root nodules of native C. korshinskii growing in arid and semi-arid region of northwester China, which were grouped into seven genospecies by 16S rRNA genes phylogenies.Ensifer, Mesorhizobium and Rhizobium accounted for 43.8%, 31.2%, 25%, respectively, and E. xinjiangensis was the predominant species. Comparing with the results of 16S rRNA genes phylogenies, their symbiotic genes had high sequence identity with each other, the incongruence between taxonomic position of symbiotic genes and 16S rRNA gene revealed the lateral gene transfer across rhizobial species or genera. |
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| Keywords: | Caragana korshinskii Rhizobia Symbiotic genes Phylogeny PCR-RFLP |
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