| 籼稻巨胚种质的诱变获得及遗传研究 |
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| 引用本文： | 章清杞,张书标,黄荣华,林光,杨仁崔.籼稻巨胚种质的诱变获得及遗传研究[J].中国农业科学,2007,40(7):1309-1314. |
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| 作者姓名： | 章清杞 张书标 黄荣华 林光 杨仁崔 |
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| 作者单位： | 1. 福建农林大学作物遗传育种研究所,福州,350002
2. 福建省闽清县农业局,闽清,350800 |
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| 摘 要： | 【目的】明确诱发产生巨胚突变的频率和巨胚基因的遗传规律。【方法】以11个籼稻品种为材料，通过60Co-γ射线辐射干种子，种植后收获自然成熟的M2代种子。将种子去壳后肉眼鉴别巨胚突变体（比原胚大至少1倍），计算水稻巨胚性状的突变频率；用通过辐射诱变获得的2个籼稻巨胚品系98-14geB和Mge86与其相应原亲本杂交配成一套F1、F2和BC1F1材料，调查种子胚的大小，研究巨胚性状的遗传。【结果】在M1代混收的M2种子中，水稻巨胚突变的出现频率平均为0.00125%，变幅为0.00029%～0.00285%。本研究以250Gy和350Gy2种剂量分别照射11个籼稻品系的干种子，在其中9个品系中获得巨胚突变体。【结论】本研究取材的2个籼稻巨胚突变体均为单隐性基因突变，且均与粳稻巨胚品种1137的巨胚基因等位，无非等位巨胚基因发现；胚的大小直接由种子基因型决定，而同为胚发育提供营养的母体基因型及细胞质均无关系。
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| 关 键 词： | 籼稻 巨胚 辐射诱变 突变率 遗传分析 |
| 收稿时间： | 2006-8-8 |
| 修稿时间： | 2006-08-082006-10-09 |
Induced Mutation and Genetic Analysis of Giant Embryo in Indica Rice |
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| ZHANG Qing-qi,ZHANG Shu-biao,HUANG Rong-hua,LIN Guang,YANG Ren-cui.Induced Mutation and Genetic Analysis of Giant Embryo in Indica Rice[J].Scientia Agricultura Sinica,2007,40(7):1309-1314. |
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| Authors: | ZHANG Qing-qi ZHANG Shu-biao HUANG Rong-hua LIN Guang YANG Ren-cui |
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| Institution: | 1.Institute of Genetics and Crop Breeding, Fujian Agriculture and Forestry University, Fuzhou 350002 ; 2. Agriculture Bureau of Minqing County, Fujian Province, Minqing 350800 |
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| Abstract: | Seeds of several indica rice cultivars were treated by nucleus irradiation and strains with giant embryo(ge) were selected in M2 and M3 generation. The inheritance of giant embryo character in rice was studied by investigating the size of embryo of plants in generations of P1、P2、F1、F2 and BC1F1, which were made by crossing the giant embryo mutants 98-14geB and Mge86 with their corresponding original types.The main results were as following: The giant embryo mutation frequency in M2 averaged 0.00125%, at range of 0.00029%-0.00285%. The giant embryo mutation frequency in M1 averaged 0.03015% or 0.03911%, depend on the different calculation methods. The ge mutants were detected in 9 M2 populations of 11 induced varieties. Mutation frequency varied with applied dosage and mutated materials. The trait of giant embryo is genetically controlled by a pair of recessive genes. The size of rice embryo is only controlled by the genotype of zygote, and not affected by the genotypes of the female parent plant and the cytoplasm. Hence, it is possible to identify giant embryo mutants directly from seeds of M2 or F2.. Population allelic tests shown that the gene in TgeB、1813geB、98-14geB and Mge86 are allelic to the ge gene of Japonica mutant “1137”. |
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| Keywords: | lndica rice Giant embryo Radiate induced mutation Mutation frequency Genetic analysis |
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