| 大蒜发芽叶培养体细胞胚发生 |
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| 引用本文： | 王洪隆,康玉庆.大蒜发芽叶培养体细胞胚发生[J].华北农学报,1994,9(1):92-94. |
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| 作者姓名： | 王洪隆 康玉庆 |
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| 作者单位： | 天津教育学院中心实验室，法国国家农业科学院蒙彼利埃研究中心 |
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| 摘 要： | 将大蒜发芽叶培养于ＭＳ（１／２ＮＨ４ＮＯ３＿＋２，４－Ｄ２ｍｇ／Ｌ＋ＫＴ０．５ｍｇ／Ｌ上，形成愈伤组织。愈伤组织继代后，转移到ＭＳ（ＫＮＯ３２５２５ｍｇ／Ｌ＋（ＮＨ４）２ＳＯ４１６５０ｍｇ／Ｌ，无ＮＨ４ＮＯ３）＋ＫＴ２ｍｇ／Ｌ＋６－ＢＡ４ｍｇ／Ｌ＋Ａｄｅｎｉｎｅ２ｍｇ／Ｌ＋ＩＡＡ０．０１ｍｇ／Ｌ培养基上，２５天后，形成体细胞胚并能再生植株。其中，胚状体发生的必要条件是ＮＯ３／ＮＨ＾＋４＞１，ＫＴ／
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| 关 键 词： | 大蒜 发芽叶 体细胞 胚 发生 |
Embryogenesis via Culture of Garlic Sprout Leaf |
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| Wang Honglong,Kang Yuqing,Zhang Cunjin.Embryogenesis via Culture of Garlic Sprout Leaf[J].Acta Agriculturae Boreali-Sinica,1994,9(1):92-94. |
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| Authors: | Wang Honglong Kang Yuqing Zhang Cunjin |
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| Abstract: | Calli appeared from sprout leaf of garlic culture on MS (1 / 2NH4NO3)+2,4-D 2mg / L+KT 0.5mg / L.These calli were survived on subculture media 4 times. Embryogenesis and plantlet regeneration occured on MS(KNO32525mg/ L+(NH4)2SO4 1650mg / L,no NH4NO3)+KT 2mg / L+6-BA 4mg / L+Adenine 2mg / L+IAA 0.01mg / L.Higher concentration of cytokinin / auxin, NO3 / NH4 >1, IAA< 0.01mg / L were essential to the embryogenesis. |
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| Keywords: | Garlic Sprout leaf Embryogenesis |
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