| 杧果主要品种遗传多态性的AFLP标记研究 |
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| 引用本文： | 雷新涛,王家保,徐雪荣,林顺权.杧果主要品种遗传多态性的AFLP标记研究[J].园艺学报,2006,33(4):725-730. |
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| 作者姓名： | 雷新涛 王家保 徐雪荣 林顺权 |
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| 作者单位： | (中国热带农业科学院南亚热带作物研究所, 广东湛江524091; 中国热带农业科学院热带生物技术研究所, 海南海口571101; 华南农业大学园艺生物技术研究所, 广东广州510640) |
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| 摘 要： | 采用扩增片段长度多态性AFLP标记对国内外31个杧果主要品种进行分类与亲缘关系研究。结果表明: 筛选出的14对引物组合在31份杧果种质中共扩增出了1 761条带, 其中多态性带的比例为97%。平均每对引物产生125.8条带和121.6条多态性带, 总的多态性带率为97%; 基于AFLP标记, 以0.48的相似系数为阈值, 所有供试 果材料被分为7大组群, 第一组群内以0.52为阈值, 又可分为6组。与形态标记相比, 基于AFLP标记的杧果分类体系更能反映杧果品种间的亲缘关系。
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| 关 键 词： | 杧果 遗传多态性 AFLP |
| 文章编号： | 0513-353X（2006）04-0725-06 |
| 收稿时间： | 2005-08-24 |
| 修稿时间： | 2005-08-242006-01-18 |
Studies on Genetic Polymorphism of Main Mango Cultivars via AFLP Markers |
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| Lei Xintao,Wang Jiabao,Xu Xuerong,Lin Shunquan.Studies on Genetic Polymorphism of Main Mango Cultivars via AFLP Markers[J].Acta Horticulturae Sinica,2006,33(4):725-730. |
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| Authors: | Lei Xintao Wang Jiabao Xu Xuerong Lin Shunquan |
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| Institution: | (Southern Subtropical Crops Research Institute, Chinese Academy of Tropical Agriculture Sciences, Zhanjiang, Guangdong 524091,China; Institute of Tropical Bioscience, Chinese Academy of Tropical Agriculture Sciences, Haikou, Hainan 571101, China; Institute of B iotechnology in Horticultural Plants, South China Agriculture University, Guangzhou, Guangdong 510640, China) |
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| Abstract: | In this article, the classification and the relationships of 31 main domestic and overseas mango(Mangifera indica Linn.)cultivars were investigated via amplified fragments length polymorphism(AFLP) markers. The results showed that the 14-pair-primer combination screened totally amplified 1 761 bands from the sampled mango cultivars, and 97% of all bands were polymorphic. For each pair of primers, there were 125.8 bands and 121.6 polymorphic bands and the polymorphic bands rate was 97%. Based on the AFLP markers, all the materials were divided into 7 groups with the threshold of 0.48 of similarity coefficient. At the same time, the first group was divided into 6 subgroups with the threshold of 0.52 of similarity coefficient. Compared with morphologic markers, the mango classification system based on AFLP markers could reflect relationship among mango cultivars more efficiently. |
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| Keywords: | Mango Genetic polymorphism AFLP |
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