| 粘虫幼虫对苏云金芽孢杆菌伴孢晶体低度敏感的机理探讨 |
| |
| 引用本文： | 邵宗泽,喻子牛.粘虫幼虫对苏云金芽孢杆菌伴孢晶体低度敏感的机理探讨[J].中国生物防治,1999,15(2):66-69. |
| |
| 作者姓名： | 邵宗泽 喻子牛 |
| |
| 作者单位： | 华中农业大学微生物科学技术系,农业微生物农业部重点室,武汉,430070 |
| |
| 摘 要： | 比较了粘虫和家蚕对苏云金芽孢杆菌库斯塔克亚种菌株ＨＤ┐１的伴孢晶体在幼虫中肠液中的体外活化过程。ＳＤＳ┐ＰＡＧＥ结果表明，伴孢晶体或溶解的１３０ｋＤａ原毒素在粘虫中肠液中活化后形成的毒性肽的浓度比家蚕低，原因是１３０ｋＤａ原毒素在粘虫中肠液中发生了过度降解。蛋白酶活性检测结果表明，粘虫中肠液中含有较高的蛋白酶活性。
|
| 关 键 词： | 苏云金芽孢杆菌 伴孢晶体 粘虫 家蚕 中肠液 |
| 修稿时间： | :1998-09-0 |
Mechanism of Low Sensitivity of Mythimna separata Larvae to Parasporal Crystals of Bacillus thuringiensis |
| |
| SHAO Zong-ze,YU Zi-niu.Mechanism of Low Sensitivity of Mythimna separata Larvae to Parasporal Crystals of Bacillus thuringiensis[J].Chinese Journal of Biological Control,1999,15(2):66-69. |
| |
| Authors: | SHAO Zong-ze YU Zi-niu |
| |
| Abstract: | The processing of parasporal crystals of Bacillus thuringiensis subsp. kurstaki HD1 in the midgut juice of Mythimna separata was studied in contrast with that of Bombyx mori SDSPAGE Results showed that both parasporal crystals and dissolved protoxins were excessively degraded in Mseparata midgut juice and only little activated toxin was generated While a larger amount of activated toxin was produced by the midgut juice of Bmori shown by SDSPAGE Further studies revealed that the midgut juice of Mseparata had a higher proteolytic activity than that of Bmori which was determined by examining their caseinhydrolyzing velocities at pH10 In addition, Mseparata midgut juice showed a more complex protein profile Our results suggested that the high proteolytic activity in Mseparata midgut juice might contribute to the low sensitivity of the larva to Bthuringiensis |
| |
| Keywords: | Bacillus thuringiensis Mythimna separata parasporal crystals Bombyx mori midgut juice |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
