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| 猪口蹄疫病毒对三种动物细胞嗜性的研究 | |
| 引用本文： | 吴锦艳,刘湘涛,胡永浩,尚佑军,田宏,孙世琪,尹双辉,冯霞.猪口蹄疫病毒对三种动物细胞嗜性的研究[J].甘肃农业大学学报,2005,40(3):306-310. |
| 作者姓名： | 吴锦艳 刘湘涛 胡永浩 尚佑军 田宏 孙世琪 尹双辉 冯霞 |
| 作者单位： | 1. 甘肃农业大学动物医学院甘肃兰州730070中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室甘肃兰州730046 2. 中国农业科学院兰州兽医研究所农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室甘肃兰州730046 3. 甘肃农业大学动物医学院甘肃兰州730070 |
| 基金项目： | 973项目(课题5:分子遗传衍化关系研究) |
| 摘 要： | 将猪FMDV毒株(O/GD/MSH/86)分别适应牛肾细胞(Bovinekidney,MD-BK)、幼仓鼠肾细胞(Babyhamsterkidney,BHK-21)和猪肾细胞(Porcinekidney,PK-15),研究了FMDV在宿主细胞上的嗜性。结果发现,该毒株在MD-BK细胞上不产生细胞病变(CPE),从细胞液中也未检测到病毒,而在BHK-21和PK-15细胞上能够稳定产生CPE。可见,猪FMDVO/GD/MSH/86毒株不能在MD-BK细胞上增殖,而在BHK-21和PK-15细胞上则增殖较好。 |
| 关 键 词： | 猪 口蹄疫病毒 细胞 嗜性 |
| 文章编号： | 1003-4315(2005)03-0306-05 |
| 修稿时间： | 2005年1月17日 |
Host tropism of swine foot-and-mouth disease virus in three animal cells |
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| WU Jinyan,Liu Xiangtao,HU Yonghao,SHANG Youjun,TIAN Hong,SUN Shiqi,YIN Shuanghui,FENG Xia.Host tropism of swine foot-and-mouth disease virus in three animal cells[J].Journal of Gansu Agricultural University,2005,40(3):306-310. | |
| Authors: | WU Jinyan Liu Xiangtao HU Yonghao SHANG Youjun TIAN Hong SUN Shiqi YIN Shuanghui FENG Xia |
| Institution: | WU Jin-yan1 2,LIU Xiang-tao2,HU Yong-hao1,SHANG You-jun2,TIAN Hong2,SUN Shi-qi2,YIN Shuang-hui2,FENG Xia2 |
| Abstract: | Swine foot-and-mouth disease virus﹙FMDV﹚﹙O/GD/MSH/86﹚were adapted to different cells, MDBK, BHK-21 and PK-15, the host tropism of FMDV was studied. The results showed that no cytopathic effect﹙CPE﹚was detected in MDBK cells, and no virus was determined in cell sap, but the virus could induce stable CPE in BHK-21 and PK-15 cells. Therefore, the swine FMDV couldn’t multiply in MDBK cells, but it could reproduction well in BHK-21 and PK-15 cells. |
| Keywords: | swine foot-and-mouth disease virus cell tropism |
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