| HPLC在黄芡降糖片质量控制中的应用 |
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| 引用本文： | 张成义,左红香,孙晓萌,金勇.HPLC在黄芡降糖片质量控制中的应用[J].吉林林学院学报,2010(5):424-426. |
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| 作者姓名： | 张成义 左红香 孙晓萌 金勇 |
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| 作者单位： | [1]北华大学药学院,吉林吉林132013 [2]延边大学药学院,吉林吉林133000 |
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| 基金项目： | 吉林省科技发展计划项目（200505248） |
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| 摘 要： | 目的建立黄芡降糖片的准确定量质控方法.方法采用高效液相色谱法（HPLC）,以YMC-C18（250 mm×4.6 mm,5μm）为色谱柱,以乙腈-水（48∶52）（含0.34%磷酸二氢钾和0.17%十二烷基硫酸钠）为流动相,流速为1.0 mL/min,检测波长为265 nm,柱温为25℃,测定黄芡降糖片中主要成分盐酸小檗碱的含量.结果盐酸小檗碱在0.64～6.40μg内线性关系良好,r=0.999 5,加样回收率为99.86%,RSD=1.01%.结论该方法操作简单、方便,重现性好,用于控制黄芡降糖片的含量,可信度高.
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| 关 键 词： | 黄连 盐酸小檗碱 高效液相色谱法 |
Application of HPLC in Quality Control of HQ |
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| Authors: | ZHANG Cheng-yi ZUO Hong-xiang SUN Xiao-meng JIN Yong |
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| Institution: | 1.Pharmacy College of Beihua University,Jilin 132013,China;2.Pharmacy College of Yanbian University,Yanbian 133000,China) |
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| Abstract: | Objective To establish an accurate method used for the control in quantity and quality of Huangqian（HQ）.Method HPLC method was used for the determination of Berberine hydrochloride in HQ on YMC-C18（250 mm×4.6 mm,5 μm）chromatographic column;acetonitrile-water（48∶52）（including 0.34% potassium phosphate dibasic solution and 0.17% sodium dodecyl sulfate） was mobile phase;the wavelength was 265 nm;the flow rate was 1.0 mL/min and the column temperature was 25 ℃.Results The linear range of Berberine hydrochloride in HQ was 0.64～6.40 μg,r=0.999 5,the average recovery was 99.86%,RSD=1.01%.Conclusion A simple,accurate and excellent in reappearance method to Berberine hydrochloride in HQ was established.It is reliable to use it to control content of Berberine hydrochloride in HQ. |
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| Keywords: | coptidis rhizoma berberine hydrochloride HPLC |
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