| 不同苗龄接穗西瓜嫁接体愈合的组织细胞学研究 |
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| 引用本文： | 张红梅,丁明,姜武,段青青,唐东梅,黄丹枫.不同苗龄接穗西瓜嫁接体愈合的组织细胞学研究[J].园艺学报,2012,39(3):493-500. |
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| 作者姓名： | 张红梅 丁明 姜武 段青青 唐东梅 黄丹枫 |
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| 作者单位： | 1上海交通大学农业与生物学院，上海 200240；2上海市农业科学院园艺研究所，上海 201106 |
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| 基金项目： | 上海市科技兴农重点攻关项目(农科攻字2002第4-3-4号);国家‘863’计划课题(2012AA101903);上海市科委科技攻关项目(11dz1960200) |
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| 摘 要： | 对不同苗龄接穗的西瓜（葫芦砧木）嫁接苗的嫁接体愈合组织进行组织学和细胞学观察。结果表明：接穗苗龄5 d、7 d和10 d的嫁接苗，嫁接愈合快，嫁接6 d后管状分子已开始分化，8 d后隔离层基本消失，维管束桥形成，近愈合面细胞内有丰富细胞器，嫁接苗的成活率达90%以上；而接穗苗龄为13 d和18 d的嫁接组合在嫁接8 d后愈合面隔离层仍存在，愈合面两侧细胞中细胞器少，鲜见维管束桥连通，嫁接苗的成活率分别为83.3%和72%。因此认为，维管束桥的形成及愈合面细胞中丰富的细胞器，是西瓜嫁接苗成活的组织学和细胞学特征，接穗苗龄小于10 d适合于进行嫁接，嫁接苗成活率高。
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| 关 键 词： | 西瓜 接穗 苗龄 嫁接体 维管束 超微结构 |
| 收稿时间： | 2011-12-19 |
Histological and Cytological Research on Graft Union Formation of Grafted Watermelon Seedlings with Different Scion Ages |
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| ZHANG Hong-mei,DING Ming,JIANG Wu,DUAN Qing-qing,TANG Dong-mei,and HUANG Dan-feng.Histological and Cytological Research on Graft Union Formation of Grafted Watermelon Seedlings with Different Scion Ages[J].Acta Horticulturae Sinica,2012,39(3):493-500. |
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| Authors: | ZHANG Hong-mei DING Ming JIANG Wu DUAN Qing-qing TANG Dong-mei and HUANG Dan-feng |
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| Institution: | 1School of Agriculture and Biology，Shanghai Jiao Tong University，Shanghai 200240，China；2Horticultural Research Institute，Shanghai Academy of Agricultural Sciences，Shanghai 201106，China |
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| Abstract: | Watermelon seedlings of different ages were grafted onto cucurbit rootstocks. The graft union formation was observed using paraffin section and a transmission electron microscope(TEM),and the survival rate was statistically analyzed. In the early days after grafting,a necrotic layer was induced between scion and rootstock. The concrescence rate of the graft combinations with 5 d,7 d and 10 d scions were higher,the necrotic layer disappeared on the 8th day after grafting,and abundant organelles occurred in the cells adjacent to the necrotic layer. Vascular bundles began to regrow after 6 days of grafting,and vascular bridges were formed after 8 days of grafting. The survival rates of these three combinations reached more than 90%. By contrast,in the graft combinations with 13 d- and 18 d-old scions,the necrotic layer could still be observed on the 8th day after grafting,and few organelles and vascular bridges were formed in the cells adjacent to the necrotic layer. The survival rates of seedlings with 13 d- and 18 d-old scions were 83.3% and 72%,respectively. Thus,the connection of vascular bridges between rootstock and scion,and abundant organelles in the cell adjacent to the necrotic layer could be considered as morphological signs of grafted seedling survival. The scion age below 10 d was appropriate for grafting. |
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| Keywords: | watermelon Citrullus lanatus graft union scion age vascular bundle ultrastructure |
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