| 钙素对SA诱导番茄幼苗抗灰霉病的调控作用 |
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| 引用本文： | 李琳琳,李天来,余朝阁,张抗抗.钙素对SA诱导番茄幼苗抗灰霉病的调控作用[J].园艺学报,2012,39(2):273-280. |
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| 作者姓名： | 李琳琳 李天来 余朝阁 张抗抗 |
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| 作者单位： | 沈阳农业大学园艺学院，设施园艺省部共建教育部重点实验室，辽宁省设施园艺重点实验室，沈阳 110866 |
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| 基金项目： | 国家‘十二五’科技支撑计划项目(2011BAD12B03);辽宁省重大科技攻关项目(2010215003);国家现代农业产业体系建设专项(Nycytx-35-gw23) |
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| 摘 要： | 为探索钙对水杨酸（SA）诱导番茄（Solanum lycopersicum）幼苗抗灰霉病的作用，采用‘L402’番茄品种，通过分别喷施8 mmol ? L-1 CaCl2和5 mmol ? L-1 EGTA后再喷施2 mmol ? L-1 SA，3 d后接种灰霉病菌孢子的方法，研究了不同处理对番茄幼苗灰霉病病情指数、活性氧积累、主要防御酶活性及其基因表达的影响。结果表明：接种灰霉病菌孢子5 d后，SA处理的番茄幼苗病情指数比对照降低37.27%，Ca + SA处理较SA处理进一步降低18%；接种1 d和2 d后，叶片中产生速率和H2O2含量分别出现应激高峰，且处理间存在差异，与对照相比Ca + SA处理分别提高33.05%和29.31%，EGTA + SA处理分别降低32.62%和46.34%；叶片中抗病相关酶活性和基因表达在接种病菌后也出现应激高峰，其中Ca + SA处理显著提高了PAL、几丁质酶、β–1,3–葡聚糖酶活性，EGTA处理及EGTA + SA处理显著降低了PAL、几丁质酶、β–1,3–葡聚糖酶活性。上述结果表明，Ca2+在SA诱导番茄抗灰霉病中具有正调控作用，而且这种作用与PAL、几丁质酶、β–1,3–葡聚糖酶活性及其基因表达密切相关。
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| 关 键 词： | 番茄 灰霉病 钙 水杨酸 诱导抗病性 调控 |
| 收稿时间： | 2011-09-02 |
The Effect of Calcium on Regulation of SA-induced Resistance to Botrytis cinerea in Tomato |
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| LI Lin-lin,LI Tian-lai,YU Chao-ge,and ZHANG Kang-kang.The Effect of Calcium on Regulation of SA-induced Resistance to Botrytis cinerea in Tomato[J].Acta Horticulturae Sinica,2012,39(2):273-280. |
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| Authors: | LI Lin-lin LI Tian-lai YU Chao-ge and ZHANG Kang-kang |
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| Institution: | College of Horticulture，Shenyang Agricultural University，Key Laboratory of Protected Horticulture Liaoning Provincial，Key Laboratory of Protected Horticulture，Ministry of Education，Shenyang 110866，China |
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| Abstract: | In order to explore the effect of calcium on the SA-induced resistance to Botrytis cinerea in tomato(Solanum lycopersicum).Tomato Botrytis cinerea susceptible strain’L402’was pretreated with foliar spraying 8 mmol·L1 CaCl2 and 5 mmol·L-1EGTA,respectively.Then followed by application of exogenous 2 mmol·L-1 SA,3 d before B.cinerea infection.The disease index,ROS accumulation,variations of the activities of main resistance enzymes and their gene expression were analyzed.The results show that the disease index is significantly reduced by 37.27%compared to control in SA treatment 5 d after B.cinerea infection;While in Ca+SA treatment,the disease index reduced by 18%compared to SA treatment.Theproduction rate and H2O2 content reached the highest level at 1 d and 2 d,respectively,there is difference among treatments:production rate ofand H2O2 content in tomatoes treated by Ca+ SA are 33.05%and 29.31%higher than contro(lH2O),however,under EGTA+SA treatment significantly reduced by 32.62%and 46.34%,respectively.The peak of disease defense-related enzymes activity and gene expression also exist,difference obtained among treatments:PAL,chintase andβ-1,3-glucancse treated by Ca+SA are significantly higher than control,whereas EGTA and EGTA+SA significantly suppress PAL,chitinase,β-1,3-glucanase activity.These results indicate that Ca2 +plays a positive regulatory role in the SA-induced resistance to Botrytis cinerea in tomato,which is closely related to the PAL,chitinase,β-1,3-glucanase activity and gene expression. |
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| Keywords: | tomato Botrytis cinerea calcium salicylic acid induce resistance regulation |
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